1,25(OH)2D3 regulates protein kinase C activity through two phospholipid-dependent pathways involving phospholipase A2 and phospholipase C in growth zone chondrocytes

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
V L SylviaB D Boyan

Abstract

We have previously shown that 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) plays a major role in growth zone chondrocyte (GC) differentiation and that this effect is mediated by protein kinase C (PKC). The aim of the present study was to identify the signal transduction pathway used by 1,25(OH)2D3 to stimulate PKC activation. Confluent, fourth passage GC cells from costochondral cartilage were used to evaluate the mechanism of PKC activation. Treatment of GC cultures with 1,25(OH)2D3 elicited a dose-dependent increase in both inositol-1,4,5-trisphosphate and diacylglycerol (DAG) production, suggesting a role for phospholipase C and potentially for phospholipase D. Addition of dioctanoylglycerol to plasma membranes isolated from GCs increased PKC activity. Neither pertussis toxin nor choleratoxin had an inhibitory effect on PKC activity in control or 1,25(OH)2D3-treated GCs, indicating that neither Gi nor Gs proteins were involved. Phospholipase A2 inhibitors, quinacrine, OEPC (selective for secretory phospholipase A2), and AACOCF3 (selective for cytosolic phospholipase A2), and the cyclooxygenase inhibitor indomethacin decreased PKC activity, while the phospholipase A2 activators melittin and mastoparan increased PKC activity in GC c...Continue Reading

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Citations

Sep 30, 2014·The Journal of Steroid Biochemistry and Molecular Biology·Maryam DoroudiZvi Schwartz
Jun 3, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H A PedrozoB D Boyan
Jul 14, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S MaedaB D Boyan
Mar 26, 2002·Molecular and Cellular Endocrinology·Moisés A Rivera-BermúdezWilliam S Mellon
Nov 15, 2002·Molecular and Cellular Endocrinology·Anthony W NormanHelen L Henry
May 2, 2002·The Journal of Steroid Biochemistry and Molecular Biology·Z SchwartzB D Boyan
Apr 30, 2010·Journal of Physiology and Biochemistry·Carmen Castaneda-Sceppa, Francisco Castaneda
Apr 15, 2016·Nutrients·Kevin B HadleyNorman Salem
Jan 2, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J SelaZ Schwartz
Jul 5, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·B D BoyanZ Schwartz
Mar 3, 2004·Journal of Cellular Biochemistry·Mathew T MizwickiAnthony W Norman
Sep 26, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Barbara D BoyanZvi Schwartz
Jul 21, 2000·The Journal of Clinical Endocrinology and Metabolism·C CrescioliM Serio
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Oct 12, 2001·The Journal of Steroid Biochemistry and Molecular Biology·V L SylviaZ Schwartz
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Dec 31, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Khairat ElBaradieZvi Schwartz
Apr 10, 2012·The Journal of Steroid Biochemistry and Molecular Biology·Maryam DoroudiBarbara D Boyan

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