PMID: 2496664May 15, 1989Paper

12S,19- and 12S,20-dihydroxyeicosanoids: novel 12S-hydroxy-5,8-cis-10-trans-14-cis-eicosatetraenoic acid metabolites formed by hydroxylation and reduction in murine lymphocytes

Archives of Biochemistry and Biophysics
R M DanilowiczT E Eling

Abstract

Murine spleen cells and purified B lymphocytes oxidized arachidonic acid via the lipoxygenase pathway. The major metabolite of both the whole spleen and enriched B lymphocytes was 12S-hydroxy-5,8-cis-10-trans-14-cis-eicosatetraenoic acid. A novel metabolite was observed that did not have an absorbance from 210 to 400 nm, indicating the absence of a conjugated double bond system. The new metabolite was converted to the methyl ester, reduced by platinum oxide, derivatized to the trimethylsilyl ether, and analyzed by gas chromatography-mass spectrometry. A major and a minor component were observed in the analysis of the new compound. The major component had major diagnostic ions indicating the presence of hydroxyl groups at C-12 and C-19. The minor component had major diagnostic ions indicating the presence of hydroxyl groups at C-12 and C-20. The new metabolites are characterized as a mixture of 12S,19- and 12S,20-dihydroxyeicosanoids presumably formed by hydroxylation and reduction of one or more double bonds of 12S-hydroxy-5,8-cis-10-trans-14-cis-eicosatetraenoic acid. These metabolites were formed predominantly with whole spleen lymphocytes but could be detected at longer incubation times or by using 12S-hydroxy-5,8-cis-10-tra...Continue Reading

References

Aug 28, 1979·Biochemical and Biophysical Research Communications·M I SiegelP Cuatrecasas
Feb 20, 1975·Biochimica Et Biophysica Acta·D H Nugteren
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R J SobermanK F Austen
Apr 29, 1986·Biochemical and Biophysical Research Communications·W S Powell
Jun 30, 1987·Biochemical and Biophysical Research Communications·W S Powell
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M HambergB Samuelsson

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