13 C APSY-NMR for sequential assignment of intrinsically disordered proteins

Journal of Biomolecular NMR
Maria Grazia MurraliIsabella C Felli

Abstract

The increasingly recognized biological relevance of intrinsically disordered proteins requires a continuous expansion of the tools for their characterization via NMR spectroscopy, the only technique so far able to provide atomic-resolution information on these highly mobile macromolecules. Here we present the implementation of projection spectroscopy in 13C-direct detected NMR experiments to achieve the sequence specific assignment of IDPs. The approach was used to obtain the complete backbone assignment at high temperature of α-synuclein, a paradigmatic intrinsically disordered protein.

References

Mar 22, 2001·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A Buevich, J Baum
Jan 30, 2003·Journal of the American Chemical Society·Seho Kim, Thomas Szyperski
Aug 13, 2003·Journal of Biomolecular NMR·Vladislav Yu OrekhovMartin Billeter
May 20, 2004·Journal of the American Chemical Society·Eriks Kupce, Ray Freeman
Jun 9, 2005·Protein Expression and Purification·Chunjuan HuangChih-chen Wang
Jul 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sebastian HillerGerhard Wider
Mar 23, 2006·Journal of the American Chemical Society·Wolfgang BermelRoberta Pierattelli
Jun 23, 2006·Journal of Biomolecular NMR·Francesco FioritoKurt Wüthrich
Oct 13, 2006·Journal of Biomolecular NMR·Krzysztof KazimierczukIgor Zhukov
Jun 25, 2008·Annual Review of Biophysics·Vladimir N UverskyA Keith Dunker
Oct 9, 2008·Journal of Biomolecular NMR·Sebastian HillerKurt Wüthrich
Mar 25, 2009·Journal of Magnetic Resonance·Wolfgang BermelPeter Tompa
Oct 3, 2009·Journal of the American Chemical Society·Wolfgang BermelRoberta Pierattelli
Nov 3, 2009·Journal of Magnetic Resonance·Anna Zawadzka-KazimierczukWiktor Koźmiński
Aug 7, 2010·Journal of the American Chemical Society·Rhagavendran L NarayananMarkus Zweckstetter
Mar 23, 2011·Journal of Biomolecular NMR·Jiří NováčekVladimír Sklenář
Jun 29, 2011·Topics in Current Chemistry·Sebastian Hiller, Gerhard Wider
Feb 22, 2012·Journal of Biomolecular NMR·Anna Zawadzka-KazimierczukLibor Krásný
Jun 12, 2012·Journal of Biomolecular NMR·Wolfgang BermelJan Stanek
Jan 15, 2013·Journal of Biomolecular NMR·Wolfgang BermelSoraya Serrano
Nov 10, 2013·Journal of Biomolecular NMR·Wolfgang BermelAnna Zawadzka-Kazimierczuk
Apr 18, 2014·Chemical Reviews·Johnny HabchiVladimir N Uversky
Apr 30, 2014·Chemical Reviews·Robin van der LeeM Madan Babu
May 17, 2014·Chemical Reviews·Vladimir N UverskyAndreas C Joerger
Oct 20, 2014·Journal of Biomolecular NMR·Alessandro PiaiIsabella C Felli
Dec 23, 2014·Nature Reviews. Molecular Cell Biology·Peter E Wright, H Jane Dyson
Feb 12, 2015·Progress in Nuclear Magnetic Resonance Spectroscopy·Isabella C Felli, Roberta Pierattelli
Nov 10, 2015·Expert Opinion on Drug Discovery·Susmitha Ambadipudi, Markus Zweckstetter
Jan 14, 2017·Journal of the American Chemical Society·Maria BaiasLucio Frydman
Jun 21, 2017·Cellular and Molecular Life Sciences : CMLS·Gabriella T HellerMichele Vendruscolo

❮ Previous
Next ❯

Citations

Jul 16, 2019·Protein Science : a Publication of the Protein Society·Chunhua DongMichael T Woodside
Jul 12, 2019·Journal of Biomolecular NMR·D Flemming Hansen
Mar 14, 2020·Biomolecules·Do-Hyoung KimKyou-Hoon Han
Nov 18, 2018·International Journal of Molecular Sciences·Do-Hyoung Kim, Kyou-Hoon Han
Feb 17, 2019·Journal of Biomolecular NMR·Jonas FredrikssonMartin Billeter
Jan 14, 2022·Chemical Reviews·Isabella C Felli, Roberta Pierattelli

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alpha-Synuclein Aggregation (MDS)

Alpha-synucleins are small proteins that are believed to restrict the mobility of synpatic vesicles and inhibit neurotransmitter release. Aggregation of these proteins have been linked to several types of neurodegenerative diseases including dementia with Lewy bodies and Parkinson's disease. Here is the latest research on α-synuclein aggregation.