13 C metabolic flux analysis-guided metabolic engineering of Escherichia coli for improved acetol production from glycerol

Biotechnology for Biofuels
Ruilian YaoHongbo Hu

Abstract

Bioprocessing offers a sustainable and green approach to manufacture various chemicals and materials. Development of bioprocesses requires transforming common producer strains to cell factories. 13C metabolic flux analysis (13C-MFA) can be applied to identify relevant targets to accomplish the desired phenotype, which has become one of the major tools to support systems metabolic engineering. In this research, we applied 13C-MFA to identify bottlenecks in the bioconversion of glycerol into acetol by Escherichia coli. Valorization of glycerol, the main by-product of biodiesel, has contributed to the viability of biofuel economy. We performed 13C-MFA and measured intracellular pyridine nucleotide pools in a first-generation acetol producer strain (HJ06) and a non-producer strain (HJ06C), and identified that engineering the NADPH regeneration is a promising target. Based on this finding, we overexpressed nadK encoding NAD kinase or pntAB encoding membrane-bound transhydrogenase either individually or in combination with HJ06, obtaining HJ06N, HJ06P and HJ06PN. The step-wise approach resulted in increasing the acetol titer from 0.91 g/L (HJ06) to 2.81 g/L (HJ06PN). To systematically characterize and the effect of mutation(s) on the...Continue Reading

References

Aug 8, 2001·European Journal of Biochemistry·S KawaiK Murata
Apr 25, 2006·Metabolic Engineering·Maciek R AntoniewiczGregory Stephanopoulos
Jun 14, 2006·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Masanari KitagawaHirotada Mori
Nov 8, 2006·Metabolic Engineering·Maciek R AntoniewiczGregory Stephanopoulos
Nov 15, 2006·Molecular Systems Biology·Uwe Sauer
Jun 5, 2007·Biotechnology and Bioengineering·Isabelle Meynial-SallesPhilippe Soucaille
Sep 23, 2008·Applied and Environmental Microbiology·Yihui ZhuElliot Altman
May 12, 2009·Acta Biochimica Et Biophysica Sinica·Feng ShiXiaoyuan Wang
Oct 12, 2013·Bioresource Technology·Hongliang ZhuXuehong Zhang
Jun 17, 2015·Applied Microbiology and Biotechnology·Ruilian YaoXuehong Zhang
Dec 20, 2015·Trends in Biotechnology·Mark J Burk, Stephen Van Dien
Oct 30, 2016·Metabolic Engineering·Nian LiuGregory Stephanopoulos
Mar 17, 2017·Annual Review of Biochemistry·Jens Nielsen
Apr 8, 2017·Current Opinion in Biotechnology·Po-Wei ChenJames C Liao
May 10, 2017·Metabolic Engineering·Lara J JazminJamey D Young
Jul 5, 2017·Current Opinion in Biotechnology·Tong Un ChaeSang Yup Lee
Jul 7, 2017·Biotechnology for Biofuels·Mukesh SainiYun-Peng Chao
Jul 30, 2017·Biotechnology and Bioengineering·Nobuyuki OkahashiHiroshi Shimizu
Aug 15, 2017·Metabolic Engineering·Siavash PartowRadhakrishnan Mahadevan
Oct 5, 2017·Biotechnology and Bioengineering·Wei LiKa-Yiu San
Nov 7, 2017·Journal of Bioscience and Bioengineering·Yoshihiro ToyaHiroshi Shimizu

❮ Previous
Next ❯

Citations

Apr 15, 2020·PLoS Computational Biology·Pierre MillardJean-Charles Portais
Jan 14, 2020·Current Opinion in Biotechnology·Yan ChenChristopher J Petzold
May 11, 2021·The Journal of General and Applied Microbiology·Taiji YuzawaTakashi Hirasawa

❮ Previous
Next ❯

Methods Mentioned

BETA
genetic modifications
PCA
PCR

Software Mentioned

Excel
SIMCA
INCA

Related Concepts

Related Feeds

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.