1,3-Dinitrobenzene neurotoxicity - Passage effect in immortalized astrocytes.

Neurotoxicology
Laura L MaurerMartin A Philbert

Abstract

Age-related disturbances in astrocytic mitochondrial function are linked to loss of neuroprotection and decrements in neurological function. The immortalized rat neocortical astrocyte-derived cell line, DI-TNC1, provides a convenient model for the examination of cellular aging processes that are difficult to study in primary cell isolates from aged brain. Successive passages in culture may serve as a surrogate of aging in which time-dependent adaptation to culture conditions may result in altered responses to xenobiotic challenge. To investigate the hypothesis that astrocytic mitochondrial homeostatic function is decreased with time in culture, low passage DI-TNC1 astrocytes (LP; #2-8) and high passage DI-TNC1 astrocytes (HP; #17-28) were exposed to the mitochondrial neurotoxicant 1,3-dinitrobenzene (DNB). Cells were exposed in either monoculture or in co-culture with primary cortical neurons. Astrocyte mitochondrial membrane potential, morphology, ATP production and proliferation were monitored in monoculture, and the ability of DI-TNC1 cells to buffer K(+)-induced neuronal depolarization was examined in co-cultures. In HP DI-TNC1 cells, DNB exposure decreased proliferation, reduced mitochondrial membrane potential and signifi...Continue Reading

References

Sep 1, 1987·Neuropathology and Applied Neurobiology·M A PhilbertA W Brown
Jan 1, 1985·Experimental Brain Research·H BjörklundL Olson
Apr 1, 1972·Journal of Ultrastructure Research·J Lipetz, V J Cristofalo
Oct 1, 1995·Bioscience Reports·T B RogersW J Lederer
Aug 26, 1998·Experimental Neurology·H M SchipperJ R Brawer
Sep 6, 2003·Cancer Letters·Marcia R WinkAna M O Battastini
Jun 21, 2005·Annals of the New York Academy of Sciences·Marie Csete
Sep 6, 2005·Nature Genetics·Anirban MaitraAravinda Chakravarti
Dec 20, 2005·Neurobiology of Aging·Da-Ting LinJames D Lechleiter
Apr 1, 2006·Journal of Cellular Physiology·Thomas LonerganBarry Bavister
May 10, 2007·Journal of Neurochemistry·Ludmila A VolobouevaRona G Giffard
Jun 15, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jun WuJames D Lechleiter
Oct 2, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Fawzi BoumezbeurKitt F Petersen
Jul 22, 2010·Glia·Jeff R LiddellGlenda M Bishop
Mar 16, 2011·Neurotoxicology·Stephen R Steiner, Martin A Philbert
May 10, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·James A MillerMartin A Philbert

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Aging Genetics (Keystone)

This feed focuses on aging epidemiology and genetic, epigenetic, and proteomic aspects underlying aging, as well as aging- associated biomarkers. Here the latest research in this domain.

Aging-Associated Metabolic Disorders

Age is associated with many metabolic disorders including cardiovascular diseases, type 2 diabetes, stroke and heart disease. The mediators in aging process have been suggested to play a part in the cellular processes responsible for these metabolic disorders. Here is the latest research on aging-associated metabolic disorders.

Cell Aging

This feed focuses on cellular aging with emphasis on mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.

Brain Aging

Here is the latest research on intrinsic and extrinsic factors, as well as pathways and mechanisms that underlie aging in the central nervous system.

Cell Aging (Keystone)

This feed focuses on cellular aging with emphasis on the mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Astrocytes & Neurodegeneration

Astrocytes are important for the health and function of the central nervous system. When these cells stop functioning properly, either through gain of function or loss of homeostatic controls, neurodegenerative diseases can occur. Here is the latest research on astrocytes and neurodegeneration.