(13)C-metabolic flux analysis of lipid accumulation in the oleaginous fungus Mucor circinelloides

Bioresource Technology
Lina ZhaoYuanda Song

Abstract

The oleaginous fungus Mucor circinelloides is of industrial interest because it can produce high levels of polyunsaturated fatty acid γ-linolenic acid. M. circinelloides CBS 277.49 is able to accumulate less than 15% of cell dry weight as lipids, while M. circinelloides WJ11 can accumulate lipid up to 36%. In order to better understand the mechanisms behind the differential lipid accumulation in these two strains, tracer experiments with (13)C-glucose were performed with the growth of M. circinelloides and subsequent gas chromatography-mass spectrometric detection of (13)C-patterns in proteinogenic amino acids was carried out to identify the metabolic network topology and estimate intracellular fluxes. Our results showed that the high oleaginous strain WJ11 had higher flux of pentose phosphate pathway and malic enzyme, lower flux in tricarboxylic acid cycle, higher flux in glyoxylate cycle and ATP: citrate lyase, together, it might provide more NADPH and substrate acetyl-CoA for fatty acid synthesis.

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Citations

Oct 21, 2016·Frontiers in Microbiology·Katrin OchsenreitherChristoph Syldatk
Sep 1, 2017·Preparative Biochemistry & Biotechnology·Juan Daniel RivaldiHeizir F de Castro
Dec 17, 2017·Applied Microbiology and Biotechnology·Behzad Satari, Keikhosro Karimi
Sep 12, 2018·World Journal of Microbiology & Biotechnology·Lei Han, Bo Liang
Jun 12, 2020·Biotechnology for Biofuels·Zhengdong ZhangXiaoyao Xie
Apr 17, 2019·International Journal of Molecular Sciences·Md Ahsanul Kabir KhanYuanda Song
Mar 1, 2018·Journal of Agricultural and Food Chemistry·Xinyi ZanYuanda Song
Mar 18, 2020·Journal of Agricultural and Food Chemistry·Xin TangWei Chen

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