(13)C NMR spectroscopy applications to brain energy metabolism

Frontiers in Neuroenergetics
Tiago B RodriguesAnne-Karine Bouzier-Sore

Abstract

(13)C nuclear magnetic resonance (NMR) spectroscopy is the method of choice for studying brain metabolism. Indeed, the most convincing data obtained to decipher metabolic exchanges between neurons and astrocytes have been obtained using this technique, thus illustrating its power. It may be difficult for non-specialists, however, to grasp thefull implication of data presented in articles written by spectroscopists. The aim of the review is, therefore, to provide a fundamental understanding of this topic to facilitate the non-specialists in their reading of this literature. In the first part of this review, we present the metabolic fate of (13)C-labeled substrates in the brain in a detailed way, including an overview of some general neurochemical principles. We also address and compare the various spectroscopic strategies that can be used to study brain metabolism. Then, we provide an overview of the (13)C NMR experiments performed to analyze both intracellular and intercellular metabolic fluxes. More particularly, the role of lactate as a potential energy substrate for neurons is discussed in the light of (13)C NMR data. Finally, new perspectives and applications offered by (13)C hyperpolarization are described.

Citations

Sep 14, 2016·Scientific Reports·Daniel SimãoPaula M Alves
Mar 10, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Gilles BonventoEmmanuel Brouillet
Aug 16, 2016·Analytical Biochemistry·Julien ValetteFawzi Boumezbeur
Sep 27, 2016·Analytical Biochemistry·Mor Mishkovsky, Arnaud Comment
Mar 9, 2018·Nature Reviews. Neuroscience·Pierre J Magistretti, Igor Allaman
Mar 20, 2018·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Tesfaye W Tefera, Karin Borges
Nov 8, 2017·Glia·L Felipe BarrosBruno Weber
Jan 20, 2017·Neurochemical Research·Sebastián Cerdán
Jun 6, 2018·Frontiers in Nutrition·Dounia El HamraniMarie-Christine Beauvieux
Sep 20, 2015·Brain : a Journal of Neurology·Katia HardiesUNKNOWN autosomal recessive working group of the EuroEPINOMICS RES Consortium
Jun 24, 2018·Scientific Reports·Talia HarrisRachel Katz-Brull
Jan 13, 2021·Cell & Bioscience·Tesfaye Wolde TeferaKarin Borges
May 15, 2021·Frontiers in Psychiatry·Ajay SarawagiAnant Bahadur Patel

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Methods Mentioned

BETA
biopsies
nuclear magnetic resonance
Human
Genome Sequencing
NMR
acetylation
electron resonance

Software Mentioned

LCModel
ANLSH

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