13C n.m.r. studies of gluconeogenesis in rat liver suspensions and perfused mouse livers

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
S M Cohen, R G Shulman

Abstract

Our early 31P n.m.r. studies of compartmentation in suspensions of rat liver cells have been extended by following fructose-1-phosphate peaks, known to be in the cytosol, which gave the same pH as the Pi peak previously assigned to the cytosol. Gluconeogenesis have been followed from [13C]glycerol labelled at C1,3 or at C2 and from labelled [3-13C]alanine. With the glycerol substrate it was possible to follow the label into alpha-glycerophosphate and to determine its distribution in the glucose formed. To a first approximation (i.e. 90%) the glucose level could be followed from its original glycerol position, e.g. [1,3-13C]glycerol to strongly labelled positions 1, 3, 4 and 6 of glucose. Slightly more than 10% of the label was scrambled (i.e. 10% movement of C2 to C1 and ca. 10% of C1 was lost, the remainder being unchanged). These are consistent with a flux through the pentose shunt, dominated by the transketolase pathway. With [3-13C]alanine, about 14 resonances are assigned to different carbons of the intermediates beta-hydroxybutyrate, acetoacetate, lactate, pyruvate, glutamate, glutamine, asparate, as well as C2-alanine, while another 7 resonances are observed from the different anomeric carbons of glucose. The effects of ...Continue Reading

Citations

Feb 1, 1985·Quarterly Reviews of Biophysics·R DeslauriersI C Smith
Feb 1, 1986·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·M E StromskiR G Shulman
Feb 22, 2012·NMR in Biomedicine·Rex E Jeffries, Jeffrey M Macdonald
Jan 1, 1984·The American Journal of Physiology·R S Balaban
Nov 9, 1981·Biochimica Et Biophysica Acta·J K Roberts, O Jardetzky
Dec 1, 1986·Archives of Biochemistry and Biophysics·T W FanA N Lane

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