PMID: 7019909May 1, 1981Paper

13C NMR study of transamination during acetate utilization by Saccharomyces cerevisiae

Proceedings of the National Academy of Sciences of the United States of America
J A den HollanderR G Shulman

Abstract

13C NMR was used to follow the metabolism of [2- 13C]acetate and [1- 13C]acetate in aerobic suspensions of Saccharomyces cerevisiae. In the experiment with [2- 13C]acetate, the 13C label appeared first in glutamate C4 and subsequently in glutamate C2 and C3. After exhaustion of the acetate, the glutamate signals diminished and the aspartate C2 and C3 peaks increased. During a subsequent chase experiment with unlabeled acetate, the aspartate peaks decreased and the glutamate C2 and C3 peaks increased in intensity. These observations are interpreted in terms of an interplay between the glutamic-oxalacetic transaminase and Krebs cycle activity. This interpretation was confirmed by an experiment with the transaminase inhibitor 2-amino oxyacetate. During all of these experiments, we observed the formation of trehalose. The NMR gives a direct measurement of the label distribution and from that information it followed that the flows through the glyoxylate and the Krebs cycles are comparable. The intermediates citrate, succinate, fumarate, malate, phosphoenolpyruvate, 3-phosphoglycerate, and glucose 6-phosphate were identified in a 13C NMR spectrum of a perchloric acid extract taken during the metabolism of [2- 13C]acetate. Enrichment ...Continue Reading

References

Jul 13, 1979·Science·R G ShulmanJ A den Hollander
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J A den HollanderR G Shulman
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S M CohenR G Shulman
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S M CohenA C McLaughlin
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·K UgurbilR G Shulman
Oct 10, 1977·Biochemical and Biophysical Research Communications·R Rognstad
Jan 1, 1970·Archives of Biochemistry and Biophysics·P S Perlman, H R Mahler
Jul 9, 1963·Biochimica Et Biophysica Acta·H L KORNBERG

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Citations

Apr 1, 1990·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·A W Jans, R Willem
Dec 20, 1982·Life Sciences·C T Burt
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T OginoK M Herrmann
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T OginoR G Shulman
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J R DickinsonR L Baxter
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·K J NeurohrR G Shulman
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C DijkemaJ Visser
May 27, 2009·International Journal of Molecular Sciences·David W SchryerMarko Vendelin
May 1, 1991·Trends in Biochemical Sciences·R G Shulman
Aug 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Robert G Shulman, Douglas L Rothman
Dec 1, 1990·Analytical Biochemistry·P LundbergH J Vogel
Jan 14, 1986·Biochemistry·J A den HollanderR G Shulman
Feb 1, 1985·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·M R BendallR G Shulman
Jan 1, 1984·Critical Reviews in Clinical Laboratory Sciences·R Rej
Dec 30, 2020·Proceedings of the National Academy of Sciences of the United States of America·Douglas L RothmanRobert G Shulman
Jul 1, 1982·Journal of Bacteriology·J K BartonR G Shulman

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