PMID: 42910Dec 1, 1979

13C nuclear magnetic resonance studies of anaerobic glycolysis in suspensions of yeast cells

Proceedings of the National Academy of Sciences of the United States of America
J A den HollanderR G Shulman

Abstract

Anaerobic glycolysis in Saccharomyces cerevisiae has been studied by 13C NMR at 90.5 MHz. [1-13c]Glucose and [6-13C]glucose were fed to suspensions of yeast cells. Time courses for concentration changes of the starting material, of courses for concentration changes of the starting material, of the intermediate fructose 1,6-bisphosphate (Fru-P2), and of the end products, ethanol and glycerol, have been followed with 1-min time resolution. The glucose uptake was well fitted by a Michaelis-Menten model, assuming competition of alpha- and beta-glucose for the same site. The Km for the uptake was found to be 10 mM for beta-glucose and 5 mM for alpha-glucose. The concentration of Fru-P2 showed an initial oscillation before it reached a co,stant level. The 13C label, introduced only as [-13C]- or [6-13C]glucose, was observed in Fru-P2 in both the C1 and C6 positions, simultaneously. From the relative intensities of the C1 Fru-P2 and C6 Fru-P2 peaks in the presence of [1-13C]- and [6-13C]glucose, in vivo kinetic information was obtained about the aldolase-triosephosphate isomerase triangle. We found that under the conditions of these experiments the ratios of backward to forward velocities through aldolase and triosephosphate isomerase...Continue Reading

References

Jul 9, 1999·Biotechnology and Bioengineering·Ana Rute NevesHelena Santos
Dec 20, 1992·Biotechnology and Bioengineering·A J MeehanA P Koretsky
Feb 20, 1995·Biotechnology and Bioengineering·C Zupke, G Stephanopoulos
Apr 1, 2011·Journal of Biosciences·Maso RicciClaudio Rossi
Oct 1, 1987·International Journal for Parasitology·C A BehmA J Jones
Dec 20, 1982·Life Sciences·C T Burt
Nov 9, 1981·Biochimica Et Biophysica Acta·J K Roberts, O Jardetzky
Jan 1, 1991·Trends in Biochemical Sciences·F M JeffreyA D Sherry
Jun 9, 2005·FEMS Microbiology Reviews·Ana Rute NevesHelena Santos
Oct 1, 1996·Nature Structural Biology·N S BlomJ Sygusch
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T L LegertonJ D Roberts
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J A den HollanderR G Shulman
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·K J NeurohrR G Shulman
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C DijkemaJ Visser
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R D NewmarkM P Klein
May 15, 1986·European Journal of Biochemistry·K D BarrowG M Smith
Sep 30, 2000·Applied and Environmental Microbiology·M R Henn, Ignacio H Chapela
Sep 27, 2002·Applied and Environmental Microbiology·Matthew R HennIgnacio H Chapela
Oct 1, 1984·The Journal of Clinical Investigation·G I ShulmanR G Shulman
Jul 31, 1986·Biochemical and Biophysical Research Communications·J L DimicoliJ M Lhoste
Sep 14, 1984·Biochimica Et Biophysica Acta·S T OxleyI D Campbell
Jan 1, 1986·Cardiovascular and Interventional Radiology·M J Kushmerick
Jun 1, 1985·Journal of Bioenergetics and Biomembranes·M HöferG Robillard
Mar 10, 2016·Progress in Nuclear Magnetic Resonance Spectroscopy·Teresa W-M Fan, Andrew N Lane
Aug 27, 2009·Mathematical Biosciences·Amalia GastaldelliSimone Bastianoni
Jan 1, 1992·Magnetic Resonance Imaging·D LanensJ Lugtenburg
May 9, 2006·Magnetic Resonance Imaging·Pierre-Gilles HenryK Ugurbil
Dec 1, 1990·Analytical Biochemistry·P LundbergH J Vogel
Nov 5, 2002·FEMS Microbiology Reviews·Jean-Charles Portais, Anne-Marie Delort
Jun 3, 2015·FEMS Yeast Research·Sara SheibaniMichael T Greenwood
Jan 14, 1986·Biochemistry·J A den HollanderR G Shulman

Citations

Jul 13, 1979·Science·R G ShulmanJ A den Hollander
May 30, 1978·Archives of Microbiology·M Bañuelos, C Gancedo
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·K UgurbilR G Shulman
Jun 1, 1964·Biochemical and Biophysical Research Communications·A Ghosh, B CHANCE
Aug 9, 1972·Biochimica Et Biophysica Acta·J U Becker, A BETZ
Oct 11, 1974·Science·U Séquin, A I Scott
Dec 20, 1974·Molecular and Cellular Biochemistry·R Serrano, G DelaFuente
Apr 29, 1968·Biochimica Et Biophysica Acta·J van Steveninck
Aug 1, 1968·European Journal of Biochemistry·C F HerediaG DelaFuente
Feb 1, 1967·Biochimica Et Biophysica Acta·A Kotyk, A Kleinzeller
Oct 1, 1958·Biochimica Et Biophysica Acta·A SOLSC ASENSIO
Mar 1, 1965·Archives of Biochemistry and Biophysics·A BETZ, B CHANCE
Jan 1, 1958·Proceedings of the National Academy of Sciences of the United States of America·I A Rose

Related Concepts

Ethanol
In Vivo NMR Spectroscopy
Glycerin
Anhydrous Dextrose
Anaerobiosis
Glycolysis
Aldolase C
Saccharomyces cerevisiae
Hydrogen-Ion Concentration
Fructosediphosphates

Related Feeds

BRAIN Initiative Cell Census Network (BICCN)

The BRAIN Initiative Cell Census Network aims to identify and provide experimental access to the different brain cell types to determine their roles in health and disease. Discover the latest research from researchers in the BRAIN Initiative Cell Census Network here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
K UgurbilR G Shulman
Proceedings of the National Academy of Sciences of the United States of America
S M CohenA C McLaughlin
© 2020 Meta ULC. All rights reserved