13C/12C isotope fractionation of aromatic hydrocarbons during microbial degradation

Environmental Microbiology
Rainer U MeckenstockHans H Richnow

Abstract

The influence of microbial degradation on the 13C/12C isotope composition of aromatic hydrocarbons is presented using toluene as a model compound. Four different toluene-degrading bacterial strains grown in batch culture with oxygen, nitrate, ferric iron or sulphate as electron acceptors were studied as representatives of different environmental redox conditions potentially prevailing in contaminated aquifers. The biological degradation induced isotope shifts in the residual, non-degraded toluene fraction and the kinetic isotope fractionation factors alphaC for toluene degradation by Pseudomonas putida (1.0026 +/- 0.00017), Thauera aromatica (1.0017 +/- 0.00015), Geobacter metallireducens (1.0018 +/- 0.00029) and the sulphate-reducing strain TRM1 (1.0017 +/- 0.00016) were in the same range for all four species, although they use at least two different degradation pathways. A similar 13C/12C isotope fractionation factor (alphaC = 1.0015 +/- 0.00015) was observed in situ in a non-sterile soil column in which toluene was degraded under sulphate-reducing conditions. No carbon isotope shifts resulting from soil-hydrocarbon interactions were observed in a non-degrading soil column control with aquifer material under the same conditio...Continue Reading

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Citations

Sep 29, 2007·Analytical and Bioanalytical Chemistry·Michaela BlessingTorsten C Schmidt
Feb 4, 2012·Analytical and Bioanalytical Chemistry·Martin ElsnerArndt Schimmelmann
May 12, 2004·Journal of Contaminant Hydrology·Torsten C SchmidtStefan B Haderlein
Jun 20, 2003·Journal of Contaminant Hydrology·Christoph SchüthKirsten Maciejczyk
Jul 12, 2003·Journal of Contaminant Hydrology·Hans H RichnowRainer U Meckenstock
Jun 19, 2001·Current Opinion in Biotechnology·F Widdel, R Rabus
Sep 27, 2002·Applied and Environmental Microbiology·Barbara MoraschRainer U Meckenstock
Oct 31, 2002·Applied and Environmental Microbiology·Mike ManefieldMark J Bailey
Jan 7, 2003·Applied and Environmental Microbiology·Silvia A ManciniBarbara Sherwood Lollar
Aug 7, 2003·Applied and Environmental Microbiology·Max L ColemanJohn D Coates
May 7, 2004·Applied and Environmental Microbiology·Barbara MoraschRainer U Meckenstock
Feb 3, 2005·Environmental Toxicology and Chemistry·Andrea ViethHans H Richnow
May 17, 2005·Environmental Microbiology·Javier I Medina-BellverSilvia Marqués
Dec 22, 2004·Journal of Contaminant Hydrology·Rainer U MeckenstockHans H Richnow
Oct 26, 2005·Journal of Contaminant Hydrology·Jennifer R McKelvieBarbara Sherwood Lollar
Feb 2, 2015·Journal of Contaminant Hydrology·Dominik HöyngFernando Mazo D'Affonseca
Apr 17, 2015·Journal of Hazardous Materials·Zemin XuFangxing Yang
Sep 16, 2017·Environmental Science and Pollution Research International·Simon LeitnerAndrea Watzinger
Aug 13, 2013·Journal of Bacteriology·Steffen KümmelHans H Richnow
Dec 1, 2009·Environmental Microbiology Reports·Steffi HerrmannHans-Hermann Richnow
Nov 27, 2008·Environmental Science & Technology·Carsten VogtAnko Fischer
Dec 17, 2008·Environmental Science & Technology·Silvia A ManciniBarbara Sherwood Lollar
Dec 14, 2007·Environmental Science & Technology·Nicole B ToblerRené P Schwarzenbach

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