PMID: 11916393Mar 28, 2002Paper

(13)C[(13)C] 2D NMR: a novel strategy for the study of paramagnetic proteins with slow electronic relaxation rates

Journal of the American Chemical Society
Timothy E MachonkinJ L Markley

Abstract

Oxidized human [2Fe-2S] ferredoxin has a notably slow electronic relaxation rate, which precludes the observation of signals from nuclei near the iron-sulfur cluster by conventional 2D or 3D methods that utilize proton detection. We have demonstrated the utility of (13)C[(13)C]CT-COSY in identifying connectivity information from fast relaxing carbon nuclei near the paramagnetic center, including those from residues that ligate the metal center.

Citations

Mar 10, 2005·Journal of Biomolecular NMR·Ivano BertiniRoberta Pierattelli
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