PMID: 9448328Mar 14, 1998Paper

13C/31P NMR studies of glucose transport in human skeletal muscle

Proceedings of the National Academy of Sciences of the United States of America
R RousselG Bloch

Abstract

The muscle intracellular (IC) free glucose concentration and the rate of muscle glycogen synthesis were measured by using in vivo 13C and 31P NMR spectroscopy in normal volunteers under hyperinsulinemic ( approximately 300 pM) clamp conditions at the following three plasma glucose levels: euglycemia ( approximately 6 mM), mild ( approximately 10 mM), and high ( approximately 16 mM) hyperglycemia. In keeping with biopsy studies, muscle IC free glucose concentration at euglycemia (-0.03 +/- 0.03 mmol/kg of muscle, mean +/- SEM, n = 10) was not statistically different from zero. A small but statistically significant amount of IC free glucose was observed during mild and high hyperglycemia: 0.15 +/- 0.08 (n = 5) and 0.43 +/- 0.20 mmol/kg of muscle (n = 5), respectively. Muscle glycogen synthesis rate, in mmol per kg of muscle per min, was 111 +/- 11 at euglycemia (n = 10), 263 +/- 29 during mild hyperglycemia (n = 5), and 338 +/- 42 during high hyperglycemia (n = 5), these three rates being significantly different from each other. As previous in vitro and in vivo studies, these rates suggest a Km (concentration at which unidirectional glucose transport reaches half-maximal rate) of the muscle glucose transport system in the 15-25 m...Continue Reading

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Citations

Jan 22, 2000·Molecular Aspects of Medicine·C Boesch
May 22, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·M L Figueiredo-GaruttiM L M Vicentini-Paulino
Jan 29, 2000·European Journal of Clinical Investigation·A J Van Den BerghA Heerschap
Dec 25, 2010·Computer Methods in Biomechanics and Biomedical Engineering·Yael Ruschkewitz, Amit Gefen
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Feb 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·R G Shulman, D L Rothman
May 12, 2009·Molecular and Cellular Endocrinology·Yin Hoon ChewRamlan Abdul Aziz
Feb 13, 2013·NMR in Biomedicine·Atiyah YahyaPeter S Allen
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Nov 1, 2006·NMR in Biomedicine·Chris BoeschFritz Schick
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Sep 14, 1999·The American Journal of Physiology·B M JuckerG I Shulman
Sep 19, 2021·Nature Protocols·Brandon FaubertRalph J DeBerardinis

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