14-3-3 acts as an intramolecular bridge to regulate cdc25B localization and activity.

The Journal of Biological Chemistry
Nichole GilesBrian Gabrielli

Abstract

One of the major regulators of mitosis in somatic cells is cdc25B. cdc25B is tightly regulated at multiple levels. The final activation step involves the regulated binding of 14-3-3 proteins. Previous studies have demonstrated that Ser-323 is a primary 14-3-3 binding site in cdc25B, which influences its activity and cellular localization. 14-3-3 binding to this site appeared to interact with the N-terminal domain of cdc25B to regulate its activity. The presence of consensus 14-3-3 binding sites in the N-terminal domain suggested that the interaction is through direct binding of the 14-3-3 dimer to sites in the N-terminal domain. We have identified Ser-151 and Ser-230 in the N-terminal domain as functional 14-3-3 binding sites utilized by cdc25B in vivo. These low affinity sites cooperate to bind the 14-3-3 dimer bound to the high affinity Ser-323 site, thus forming an intramolecular bridge that constrains cdc25B structure to prevent access of the catalytic site. Loss of 14-3-3 binding to either N-terminal site relaxes cdc25B structure sufficiently to permit access to the catalytic site, and the nuclear export sequence located in the N-terminal domain. Mutation of the Ser-323 site was functionally equivalent to the mutation of a...Continue Reading

References

Apr 1, 1997·Current Opinion in Cell Biology·D K Morrison, R E Cutler
Jul 15, 1999·Biochemical and Biophysical Research Communications·A R ForrestB G Gabrielli
Aug 12, 1999·The Journal of Cell Biology·C KarlssonJ Pines
Mar 31, 2000·Progress in Cell Cycle Research·V Baldin
May 24, 2000·Oncogene·N DavezacB Ducommun
Jun 15, 2000·Experimental Cell Research·C P De SouzaB G Gabrielli
Jul 24, 2001·Oncogene·A Forrest, B Gabrielli
Jul 11, 2002·The Journal of Biological Chemistry·Véronique BaldinBernard Ducommun
Jan 14, 2003·Nature Cell Biology·Mark JackmanJonathon Pines
Jan 16, 2003·Oncogene·Nathalie Theis-FebvreVéronique Baldin

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Citations

Aug 31, 2010·The Journal of Biological Chemistry·Puji AstutiBrian Gabrielli
Jan 8, 2011·The Journal of Biological Chemistry·Jianying XiaoBingzhi Yu
Jan 19, 2007·Molecular Biology of the Cell·Mónica AlvarezSusana de la Luna
Nov 16, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zhe ZhangChuize Kong
Dec 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kathrin MudaFriedrich W Herberg
Jul 1, 2008·Fungal Genetics and Biology : FG & B·Natalia Mielnichuk, José Pérez-Martín
Jun 26, 2015·Journal of Bioscience and Bioengineering·Rima MatsuyamaTakeshi Omasa
Nov 28, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yang ZhangBing-Zhi Yu
Oct 29, 2014·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Xiangyu ZhaoBingzhi Yu
Mar 9, 2004·Biochemical and Biophysical Research Communications·Sanae UchidaKatsumi Yamashita
May 16, 2006·Seminars in Cancer Biology·Heiko Hermeking, Anne Benzinger
May 29, 2012·Asian Pacific Journal of Cancer Prevention : APJCP·Xiu-Yue YuChui-Ze Kong
Sep 29, 2011·Seminars in Cell & Developmental Biology·Alexandra K Gardino, Michael B Yaffe
Feb 3, 2009·Biochimica Et Biophysica Acta·Valerie LobjoisBernard Ducommun
Sep 18, 2012·Biochimica Et Biophysica Acta·Yong Hwan ParkIck Young Kim
Mar 28, 2008·Developmental Biology·Petr SolcJan Motlik
Jul 18, 2008·Biology of Reproduction·Cheng CuiBingzhi Yu
Jun 8, 2007·Biology of Reproduction·Chen FengBingzhi Yu
Jan 11, 2005·Oncogene·David L MyerPeter J Stambrook
May 5, 2006·Journal of Biochemistry·Sanae UchidaKatsumi Yamashita
Jan 13, 2018·Genes & Cancer·Cecil J GomesJesse D Martinez
Oct 12, 2019·FEBS Letters·Adrijana Crncec, Helfrid Hochegger
Sep 28, 2006·Journal of Cell Science·Estelle SchmittChristine Dozier
Apr 20, 2004·Journal of Cell Science·Michele K Dougherty, Deborah K Morrison
May 7, 2004·Journal of Cell Science·Stéphanie DutertreBernard Ducommun
Oct 1, 2004·Journal of Cell Science·Arne LindqvistChristina Karlsson Rosenthal

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