14-3-3σ controls corneal epithelium homeostasis and wound healing

Investigative Ophthalmology & Visual Science
Qingxian LuQiutang Li

Abstract

To investigate the functional role of 14-3-3σ in regulation of the corneal epithelial proliferation, differentiation, and wound-healing response. Corneal phenotypes were investigated in heterozygous repeated epilation (Er) mice carrying mutations in the sfn (14-3-3σ) gene. Immunohistochemistry was used to study the corneal morphogenesis of the Er/Er embryos at embryonic day (E)18.5. Corneal homeostasis and the wound-healing response were investigated macroscopically and microscopically in the adult heterozygous Er mice. Corneal epithelial cell proliferation and differentiation were assessed by BrdU incorporation and immunohistochemistry with specific antibodies for differentiation markers. Furthermore, corneal stroma neovascularization and meibomian gland degeneration were examined by immunohistochemistry. The healing of corneal wounds after debridement was monitored and visualized by fluorescent staining. Homozygous mutation of 14-3-3σ led to defects in embryonic corneal epithelial development and differentiation, whereas young heterozygotes showed normal corneal development and homeostasis. However, older heterozygotes displayed a dramatic corneal wound-healing defect characterized by hyperplastic basal progenitor cells (some...Continue Reading

Citations

Jul 23, 2013·PloS One·Asadolah MovahedanAli R Djalilian
Feb 11, 2015·The Journal of Investigative Dermatology·Sumitha A T SambandamQiutang Li
Mar 10, 2018·International Journal of Molecular Sciences·Sander BekeschusKristian Wende
Jul 28, 2016·Cellular and Molecular Life Sciences : CMLS·Carmen Ehrmann, Marlon R Schneider
Jan 20, 2021·Nature Communications·Mingsen LiHong Ouyang

Related Concepts

Sfn protein, mouse
Atrophic
Cell Differentiation Process
Lacrimal Gland Structure
Structure of Meibomian Gland
Mice, Inbred CBA
Mice, Inbred C57BL
Morphogenesis
Wound Healing
Western Blot

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