14-3-3 protein C-terminal stretch occupies ligand binding groove and is displaced by phosphopeptide binding.

The Journal of Biological Chemistry
Jan SilhanTomas Obsil

Abstract

14-3-3 proteins are important regulators of numerous cellular signaling circuits. They bind to phosphorylated protein ligands and regulate their functions by a number of different mechanisms. The C-terminal part of the 14-3-3 protein is known to be involved in the regulation of 14-3-3 binding properties. The structure of this region is unknown; however, a possible location of the C-terminal stretch within the ligand binding groove of the 14-3-3 protein has been suggested. To fully understand the role of the C-terminal stretch in the regulation of the 14-3-3 protein binding properties, we investigated the physical location of the C-terminal stretch and its changes upon the ligand binding. For this purpose, we have used Forster resonance energy transfer (FRET) measurements and molecular dynamics simulation. FRET measurements between Trp242 located at the end of the C-terminal stretch and a dansyl group attached at two different cysteine residues (Cys25 or Cys189) indicated that in the absence of the ligand, the C-terminal stretch occupies the ligand binding groove of 14-3-3 protein. Our data also showed that phosphopeptide binding displaces the C-terminal stretch from the ligand binding groove. Intramolecular distances calculated...Continue Reading

References

Oct 6, 1995·The Journal of Biological Chemistry·Z J LuoJ Avruch
Jan 1, 1994·Methods in Enzymology·J C Brochon
Apr 1, 1994·Analytical Biochemistry·P Wu, L Brand
Jun 14, 1996·The Journal of Biological Chemistry·Y C LiuA Altman
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Jan 17, 1997·Journal of Molecular Biology·T HanssonJ Aqvist
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·H FuS C Masters
Mar 15, 2001·Current Opinion in Cell Biology·M B Yaffe, A E Elia
Oct 3, 2002·Proteins·Amy B TruongHaian Fu
Feb 28, 2003·The EMBO Journal·Martin WürteleClaudia Oecking
May 20, 2003·The Plant Journal : for Cell and Molecular Biology·Wei ShenSteven C Huber
Oct 17, 2003·The Journal of Biological Chemistry·Houbin ZhangWolfgang Baehr
Nov 14, 2003·The Journal of Biological Chemistry·Veronika ObsilovaTomas Obsil

❮ Previous
Next ❯

Citations

May 7, 2009·The Journal of Biological Chemistry·Jan SilhanTomas Obsil
Nov 13, 2008·Plant & Cell Physiology·Sabina ViscontiPatrizia Aducci
Aug 11, 2005·Science's STKE : Signal Transduction Knowledge Environment·Dave Bridges, Greg B G Moorhead
Mar 23, 2011·Biochemistry. Biokhimii︠a︡·N N Sluchanko, N B Gusev
Aug 20, 2011·PloS One·Stephen J BrokxRaymond Hui
Aug 29, 2012·Protoplasma·Albertus H de BoerJing Gao
Jan 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Surajit GangulyDavid C Klein
Jan 29, 2009·Journal of Cellular Biochemistry·Katerina KuzelováZbynek Hrkal
Dec 20, 2005·The Plant Journal : for Cell and Molecular Biology·Mark P SinnigeAlbertus H de Boer
Sep 17, 2011·Seminars in Cell & Developmental Biology·Tomas Obsil, Veronika Obsilova
Dec 4, 2012·Biophysical Journal·Lenka RezabkovaTomas Obsil
Oct 4, 2018·The FEBS Journal·Aneta SmidovaVeronika Obsilova
May 15, 2015·The Journal of Biological Chemistry·Miroslava KacirovaTomas Obsil
Jul 1, 2020·FEBS Letters·Barbora Landová, Jan Šilhán
Sep 8, 2019·Cellular Oncology (Dordrecht)·Tiziana FiorettiGabriella Esposito
Apr 23, 2008·The Journal of Biological Chemistry·Lucas R JagemannJosé Lozano
May 29, 2020·American Journal of Physiology. Renal Physiology·Shan ChenHui Cai

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.