PMID: 9450960Apr 4, 1998Paper

14-3-3 proteins act as negative regulators of the mitotic inducer Cdc25 in Xenopus egg extracts

Molecular Biology of the Cell
A KumagaiW G Dunphy

Abstract

Cdc25, the dual-specificity phosphatase that dephosphorylates the Cdc2-cyclin B complex at mitosis, is highly regulated during the cell cycle. In Xenopus egg extracts, Cdc25 is associated with two isoforms of the 14-3-3 protein. Cdc25 is complexed primarily with 14-3-3epsilon and to a lesser extent with 14-3-3zeta. The association of these 14-3-3 proteins with Cdc25 varies dramatically during the cell cycle: binding is high during interphase but virtually absent at mitosis. Interaction with 14-3-3 is mediated by phosphorylation of Xenopus Cdc25 at Ser-287, which resides in a consensus 14-3-3 binding site. Recombinant Cdc25 with a point mutation at this residue (Cdc25-S287A) is incapable of binding to 14-3-3. Addition of the Cdc25-S287A mutant to Xenopus egg extracts accelerates mitosis and overrides checkpoint-mediated arrests of mitotic entry due to the presence of unreplicated and damaged DNA. These findings indicate that 14-3-3 proteins act as negative regulators of Cdc25 in controlling the G2-M transition.

References

Aug 1, 1992·Molecular Biology of the Cell·T IzumiJ L Maller
Apr 1, 1992·The EMBO Journal·F al-Khodairy, A M Carr
Oct 4, 1991·Cell·W G Dunphy, A Kumagai
Feb 28, 1991·Nature·C Featherstone, P Russell
Sep 5, 1991·Nature·M IgarashiH Okayama
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J B MillarP Russell
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D S ConklinD Beach
Jan 1, 1995·Molecular Biology of the Cell·P R MuellerW G Dunphy
Mar 9, 1995·Nature·D O Morgan
Dec 1, 1994·Current Opinion in Cell Biology·T R Coleman, W G Dunphy
Nov 18, 1994·Cell·R W KingM W Kirschner
Aug 1, 1996·The Journal of Cell Biology·P JinD O Morgan
Jan 1, 1997·Methods in Enzymology·A Kumagai, W G Dunphy

❮ Previous
Next ❯

Citations

Feb 13, 2008·Molecular Biotechnology·Anna Philpott, P Renee Yew
Aug 1, 2000·Mutation Research·T Humphrey
Apr 24, 1999·Biochimie·T Caspari, A M Carr
May 23, 2002·FEMS Microbiology Letters·Peter R KrausSteven D Harris
Nov 7, 2000·Current Opinion in Cell Biology·C G Takizawa, D O Morgan
Mar 15, 2001·Current Opinion in Cell Biology·M B Yaffe, A E Elia
Jan 23, 1999·Current Opinion in Cell Biology·N Rhind, P Russell
Apr 21, 1999·Current Opinion in Cell Biology·R Ohi, K L Gould
Feb 13, 2001·Current Opinion in Genetics & Development·N C Walworth
Jul 14, 2011·Nature Reviews. Molecular Cell Biology·Claudia Wurzenberger, Daniel W Gerlich
Aug 24, 2013·Nature Reviews. Molecular Cell Biology·H Christian Reinhardt, Michael B Yaffe
May 16, 2008·The Journal of Biological Chemistry·Jianping JinJ Wade Harper
Jul 17, 2013·The Journal of Cell Biology·Peng WangVincent Archambault
Apr 10, 1999·Molecular Biology of the Cell·B FurnariP Russell
Sep 3, 1999·Molecular Biology of the Cell·H M VerkadeM J O'Connell
May 4, 2000·Molecular Biology of the Cell·Z Guo, W G Dunphy
Sep 12, 2000·Molecular Biology of the Cell·N C KappasJ C Sible
Mar 17, 2001·Molecular Biology of the Cell·J LeeW G Dunphy
Oct 22, 2002·Molecular Biology of the Cell·Thomas J McGarry
Feb 10, 2006·Molecular Biology of the Cell·Seth S MargolisSally Kornbluth
Mar 19, 2002·EMBO Reports·Fumiko Toyoshima-MorimotoEisuke Nishida
Feb 16, 2002·The EMBO Journal·Caroline ClucasIain L Johnstone
Apr 15, 1999·Journal of the National Cancer Institute·W G Kaelin
Mar 11, 1999·Genes & Development·M S MurakamiG F Vande Woude
Mar 25, 1999·Genes & Development·L ChenN C Walworth
Jun 30, 2012·Journal of Gastroenterology and Hepatology·Chung-Wah WuJun Yu
May 22, 2001·Molecular and Cellular Biology·M S ChenH Piwnica-Worms
Jun 8, 2001·Molecular and Cellular Biology·H Zhao, H Piwnica-Worms
Mar 10, 2001·Molecular and Cellular Biology·N Rhind, P Russell
Jan 6, 2005·Molecular and Cellular Biology·George ZachosDavid A F Gillespie
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·H FuS C Masters
May 8, 2007·Cell Division·Jennifer A Perry, Sally Kornbluth
Feb 14, 2014·PLoS Biology·Tony Y-C TsaiJames E Ferrell
Apr 18, 2012·World Journal of Gastroenterology : WJG·Mariana Ferreira LealMarília de Arruda Cardoso Smith
Apr 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Quentin Liu, Joan V Ruderman
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·B D Aguda
Oct 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Hui ZhaoHelen Piwnica-Worms

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.