Abstract
The 14-3-3 proteins associate with many cellular proteins that participate in the regulation of various cellular events including apoptosis, the cell cycle, spreading, and migration. We have previously described that 14-3-3beta binds the beta1-integrin and overexpression of 14-3-3beta promoted increased cell spreading and migration (Han et al. [2001] Oncogene 20: 346-357). In this study, we find that mutation of Ser 60 of 14-3-3beta, outside of the amphipathic groove which is involved in 14-3-3 protein interactions with other ligands, abolished its interaction with integrin. Surprisingly, this mutant retained its ability to promote cell spreading, suggesting that 14-3-3beta interaction with the beta1-integrin is not required for its regulation of cell adhesion. We next showed that mutations of several critical residues in the amphipathic groove did not affect 14-3-3beta interaction with the beta1-integrin. As expected, these mutants disrupted their association with the phosphoserine dependent ligands Raf and Cas. Analysis of the groove mutant LF (mutation of Arg129Tyr130 to Leu and Phe) indicated that, unlike wild type 14-3-3beta, it could not stimulate cell spreading or migration, suggesting that a functional amphipathic groov...Continue Reading
References
Jun 1, 1992·European Journal of Biochemistry·A TokerA Aitken
Dec 1, 1992·Trends in Biochemical Sciences·A AitkenJ Soll
Aug 17, 1992·FEBS Letters·T IsobeK Nakaya
Jul 31, 1990·European Journal of Biochemistry·A TokerA Aitken
May 1, 1988·The Journal of Cell Biology·E E Marcantonio, R O Hynes
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·N Bonnefoy-BérardA Altman
Jul 13, 1995·Nature·D LiuR Liddington
Mar 17, 1995·The Journal of Biological Chemistry·A AitkenY Patel
Oct 13, 1994·Nature·W J FantlL T Williams
Sep 16, 1994·Science·E FreedR Ruggieri
Sep 16, 1994·Science·K IrieK Matsumoto
Nov 15, 1996·Cell·J ZhaS J Korsmeyer
Feb 21, 1997·Cell·P E HughesM H Ginsberg
May 23, 1997·The Journal of Biological Chemistry·L ZhangH Fu
Jun 1, 1997·Mechanisms of Development·C RommelE Hafen
Aug 22, 1997·The Journal of Biological Chemistry·G J ClarkS L Campbell
Oct 23, 1997·Current Opinion in Cell Biology·F G Giancotti
Jun 20, 1998·The Journal of Biological Chemistry·C PetosaR C Liddington
Aug 15, 1998·The Journal of Biological Chemistry·T MegidishS Hakomori
Sep 5, 1998·Trends in Cell Biology·P E Hughes, M Pfaff
Jan 29, 1999·Nature·A Lopez-GironaP Russell
Feb 20, 1999·The Journal of Biological Chemistry·M Garcia-GuzmanK Vuori
Aug 17, 1999·Biochemical and Biophysical Research Communications·H HondaH Hirai
Feb 8, 2000·The Journal of Biological Chemistry·K InouyeY Kaziro
Sep 22, 2000·The Biochemical Journal·M T Yip-SchneiderM S Marshall
Apr 21, 2001·Oncogene·D C HanJ L Guan
Apr 17, 2002·The Journal of Biological Chemistry·David W PowellKenneth R McLeish
Jun 22, 2002·Molecular and Cellular Biology·Yvonne LightRichard Marais
Jul 23, 2002·Molecular Biology of the Cell·Paul E HughesMark H Ginsberg
Sep 12, 2002·The Journal of Biological Chemistry·Dong Cho HanJun-Lin Guan
Jun 18, 2003·The Journal of Biological Chemistry·Katarzyna BialkowskaJoan E B Fox
Citations
Nov 12, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jerzy K KulskiHidetoshi Inoko
Apr 27, 2011·Archives of Microbiology·Daliang LuoShengli Tian
Apr 29, 2005·Cell Research·Paulette Mhawech
Nov 30, 2007·Molecular Biology of the Cell·Cédric BadowskiMarc R Block
Jun 14, 2008·Blood·Heikki TakalaSusanna C Fagerholm
Sep 4, 2007·BMC Cancer·Ajay MattaRanju Ralhan
May 7, 2008·The Journal of Cell Biology·Naoto KakinumaRyoiti Kiyama
Oct 2, 2007·British Journal of Pharmacology·C A LunnL Bober
Jun 15, 2013·Journal of Molecular Biology·Roman BonetIain D Campbell
Oct 16, 2009·The International Journal of Biochemistry & Cell Biology·Wei-Guo HuangZhi-Qiang Xiao
Apr 16, 2016·Cancer Gene Therapy·L M JinS S Meng
Apr 22, 2015·Nature Medicine·Myriam ChimenG Ed Rainger
Nov 12, 2014·International Journal of Oncology·De-Yu ChenWen-Qing Qi
Jun 17, 2006·Theriogenology·Hye-Jin JeongKwang-Yul Cha
Oct 5, 2011·The American Journal of Pathology·Tzu-An LiuJun-Yang Liou
Jul 15, 2009·BMC Research Notes·Yoonseok KamVito Quaranta
Mar 22, 2005·Molecular & Cellular Proteomics : MCP·Anne BenzingerHeiko Hermeking
May 2, 2007·The Journal of Biological Chemistry·Joonsung HwangMaria I Morasso
Mar 17, 2007·Psychiatry and Clinical Neurosciences·Kaoru SugimoriYoshifumi Koshino
Jul 28, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Chao-Zhu He, Kun-He Zhang
Dec 16, 2017·The Journal of Pathology·Colin KennyMaureen J O'Sullivan
Oct 28, 2017·Frontiers in Molecular Neuroscience·Brett Cornell, Kazuhito Toyo-Oka
Dec 12, 2019·PeerJ·Yongbin WangWenbin Li
Apr 15, 2008·Journal of Proteome Research·Ajay MattaK W Michael Siu
Apr 28, 2006·Journal of Cell Science·Vinzenz LinkCarl-Philipp Heisenberg
Jan 2, 2009·Journal of Cell Science·Kyle R Legate, Reinhard Fässler
Aug 6, 2021·Stem Cells International·Shao Yue ZhuMin Gu
Oct 1, 2010·Journal of Proteome Research·Feng GeXian-En Zhang