14-3-3εa directs the pulsatile transport of basal factors toward the apical domain for lumen growth in tubulogenesis

Proceedings of the National Academy of Sciences of the United States of America
Yuji MizotaniMasaya Imoto

Abstract

The Ciona notochord has emerged as a simple and tractable in vivo model for tubulogenesis. Here, using a chemical genetics approach, we identified UTKO1 as a selective small molecule inhibitor of notochord tubulogenesis. We identified 14-3-3εa protein as a direct binding partner of UTKO1 and showed that 14-3-3εa knockdown leads to failure of notochord tubulogenesis. We found that UTKO1 prevents 14-3-3εa from interacting with ezrin/radixin/moesin (ERM), which is required for notochord tubulogenesis, suggesting that interactions between 14-3-3εa and ERM play a key role in regulating the early steps of tubulogenesis. Using live imaging, we found that, as lumens begin to open between neighboring cells, 14-3-3εa and ERM are highly colocalized at the basal cortex where they undergo cycles of accumulation and disappearance. Interestingly, the disappearance of 14-3-3εa and ERM during each cycle is tightly correlated with a transient flow of 14-3-3εa, ERM, myosin II, and other cytoplasmic elements from the basal surface toward the lumen-facing apical domain, which is often accompanied by visible changes in lumen architecture. Both pulsatile flow and lumen formation are abolished in larvae treated with UTKO1, in larvae depleted of either...Continue Reading

References

Oct 24, 1998·Bioorganic & Medicinal Chemistry·S L Schreiber
Mar 20, 2001·Nature Reviews. Genetics·B R Stockwell
Feb 1, 2002·Developmental Biology·Takehiro KusakabeYutaka Satou
Jun 5, 2004·Developmental Cell·Ichiko SaotomeAndrea I McClatchey
Jun 5, 2004·Developmental Cell·Verena GöbelJohn T Fleming
Dec 21, 2005·Current Opinion in Cell Biology·Edwin M Munro
Jun 27, 2006·Nature·Makoto KameiBrant M Weinstein
Nov 16, 2006·Chemical Biology & Drug Design·Ryan D MurpheyLeonard I Zon
May 15, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Di Jiang, William C Smith
Jun 6, 2007·Development, Growth & Differentiation·Kohji HottaHiroki Takahashi
Jun 9, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kohji HottaKazuho Ikeo
Mar 28, 2009·Developmental Biology·Bo DongDi Jiang
Oct 27, 2009·Developmental Cell·Boris StrilićEckhard Lammert
Feb 9, 2010·Current Biology : CB·Louis Gervais, Jordi Casanova
Oct 5, 2010·Nature Cell Biology·David M BryantKeith E Mostov
Oct 19, 2010·Current Biology : CB·Qiongqiong ZhouDouglas N Robinson
Feb 9, 2011·Current Biology : CB·Anirban DattaKeith E Mostov
Feb 11, 2011·Bioorganic & Medicinal Chemistry Letters·Masato SawadaMasaya Imoto
Nov 22, 2011·Current Biology : CB·Ann M WehmanJeremy Nance
Aug 9, 2012·Trends in Cell Biology·Guillaume SalbreuxEwa Paluch
Dec 1, 2012·Nature Cell Biology·Gerard ApodacaDavid M Bryant
Dec 18, 2012·Nature Cell Biology·Bruno Thomas FievetJulie Ahringer
May 21, 2014·Cellular Logistics·Dongying LiRytis Prekeris
Jul 2, 2015·Nature Reviews. Molecular Cell Biology·Michael MurrellMargaret L Gardel
Oct 6, 2015·Scientific Reports·Bastian Rouven BrücknerAndreas Janshoff
Jun 16, 2016·F1000Research·Michael T Veeman, Jocelyn A McDonald

❮ Previous
Next ❯

Citations

Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Punit BhattachanBo Dong
Jul 14, 2020·Frontiers in Cell and Developmental Biology·Kwantae KimAlberto Stolfi
Feb 2, 2021·Trends in Pharmacological Sciences·F Sanders Pair, Talene A Yacoubian
Nov 17, 2020·Frontiers in Cell and Developmental Biology·Hongzhe PengBo Dong

❮ Previous
Next ❯

Methods Mentioned

BETA
pull down
antisense oligonucleotide
PCR
Pull-Down
Assay
immunoprecipitation
antisense oligonucleotides

Software Mentioned

MATLAB
Fiji
GeneTools

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.