14-3-3beta protein associates with insulin receptor substrate 1 and decreases insulin-stimulated phosphatidylinositol 3'-kinase activity in 3T3L1 adipocytes.

The Journal of Biological Chemistry
A KosakiH Kuzuya

Abstract

The 14-3-3 protein family has been implicated in growth factor signaling. We investigated whether 14-3-3 protein is involved in insulin signaling in 3T3L1 adipocytes. A significant amount of insulin receptor substrate 1 (IRS-1) was immunodetected in the immunoprecipitate with anti-14-3-3beta antibody at the basal condition. 100 nM insulin increased the amount of IRS-1 in the immunoprecipitate 2.5-fold. The effect of insulin was abolished by 100 nM wortmannin. An in vitro binding study revealed that glutathione S-transferase-14-3-3beta fusion protein directly associates with recombinant IRS-1. Pretreatment of recombinant IRS-1 with alkaline phosphatase clearly decreased this association. Because the recombinant IRS-1 was not phosphorylated on its tyrosine residues, the results suggest that serine/threonine phosphorylation of IRS-1 is responsible for the association. When the cells are treated with insulin, phosphatidylinositol 3'-kinase (PI3K) is supposed to complex either 14-3-3beta-IRS-1 or IRS-1. The 14-3-3beta-IRS-1-PI3K and IRS-1-PI3K complexes were separately prepared by a sequential immunoprecipitation, first with anti-14-3-3beta and then with anti-IRS-1 antibodies. The specific activity of the PI3K in the former was appr...Continue Reading

References

Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·N Bonnefoy-BérardA Altman
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D S ConklinD Beach
Mar 1, 1995·Trends in Biochemical Sciences·A Aitken
Apr 1, 1995·Endocrine Reviews·B Cheatham, C R Kahn
Oct 13, 1994·Nature·W J FantlL T Williams
Aug 1, 1995·Trends in Endocrinology and Metabolism : TEM·M G Myers, M F White

❮ Previous
Next ❯

Citations

Oct 13, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M J van HemertG P van Heusden
Sep 3, 2008·Science in China. Series C, Life Sciences·Pengcheng ZhuShouyuan Zhao
Dec 4, 2009·The Journal of Biological Chemistry·Miklós PéterfyKaren Reue
Feb 10, 2009·BMC Evolutionary Biology·Daniel J Macqueen, Ian A Johnston
Nov 30, 2006·Arthritis Research & Therapy·Frédéric MassicotteDaniel Lajeunesse
Jun 5, 2012·PloS One·Jun LiuWeihong Tan
Oct 19, 2011·Biochemical Pharmacology·R Quintana-PortilloC González-Yanes
Mar 7, 2006·Trends in Endocrinology and Metabolism : TEM·Nira Ben-JonathanChristopher R LaPensee
Oct 24, 2006·Experimental Cell Research·Merit E Reyes-ReyesSteven K Akiyama
Apr 3, 1999·The Journal of Biological Chemistry·L Y QiaoX J Sun
Apr 15, 2004·Endocrine Reviews·Robert T WatsonJeffrey E Pessin
Jul 10, 1999·The Journal of Biological Chemistry·B FernándezH Meisner
Nov 16, 2001·The Journal of Biological Chemistry·Guri Tzivion, Joseph Avruch
Mar 26, 2002·The Journal of Biological Chemistry·Hiroyuki SanoGustav E Lienhard
Sep 12, 2018·Endocrinology·Yves Mugabo, Gareth E Lim
Jun 7, 2007·The Biochemical Journal·Sara FröjdöLuciano Pirola
Oct 19, 2001·Oncogene·G TzivionJ Zhu
Jul 25, 2007·Molecular and Cellular Biochemistry·Abelardo MedinaAziz Ghahary
Aug 30, 2007·The Journal of Biological Chemistry·Ryoji NagaiNorma Frizzell
Jul 30, 1999·Biochemical and Biophysical Research Communications·K TakekoshiT Nakai
May 9, 2001·Biochimica Et Biophysica Acta·D Harrison-FindikP J Robinson
May 16, 2006·Seminars in Cancer Biology·Heiko Hermeking, Anne Benzinger

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.