14-3-3gamma mediates Cdc25A proteolysis to block premature mitotic entry after DNA damage.

The EMBO Journal
Kousuke KasaharaMasaki Inagaki

Abstract

14-3-3 proteins control various cellular processes, including cell cycle progression and DNA damage checkpoint. At the DNA damage checkpoint, some subtypes of 14-3-3 (beta and zeta isoforms in mammalian cells and Rad24 in fission yeast) bind to Ser345-phosphorylated Chk1 and promote its nuclear retention. Here, we report that 14-3-3gamma forms a complex with Chk1 phosphorylated at Ser296, but not at ATR sites (Ser317 and Ser345). Ser296 phosphorylation is catalysed by Chk1 itself after Chk1 phosphorylation by ATR, and then ATR sites are rapidly dephosphorylated on Ser296-phosphorylated Chk1. Although Ser345 phosphorylation is observed at nuclear DNA damage foci, it occurs more diffusely in the nucleus. The replacement of endogenous Chk1 with Chk1 mutated at Ser296 to Ala induces premature mitotic entry after ultraviolet irradiation, suggesting the importance of Ser296 phosphorylation in the DNA damage response. Although Ser296 phosphorylation induces the only marginal change in Chk1 catalytic activity, 14-3-3gamma mediates the interaction between Chk1 and Cdc25A. This ternary complex formation has an essential function in Cdc25A phosphorylation and degradation to block premature mitotic entry after DNA damage.

References

Nov 12, 1998·Trends in Biochemical Sciences·P Russell
Dec 2, 2000·Nature·B B Zhou, S J Elledge
May 22, 2001·Molecular and Cellular Biology·M S ChenH Piwnica-Worms
Jun 8, 2001·Molecular and Cellular Biology·H Zhao, H Piwnica-Worms
Mar 26, 2002·Nature Genetics·A Jeannine LincolnPeter J Donovan
Apr 5, 2003·The Journal of Biological Chemistry·Kecheng JiangYolanda Sanchez
Jun 5, 2003·Cancer Cell·Jiri Bartek, Jiri Lukas
Jun 7, 2003·Science·Lee Zou, Stephen J Elledge
Oct 16, 2003·Molecular and Cellular Biology·Mei-Shya ChenHelen Piwnica-Worms
Dec 19, 2003·Genes & Development·Jianping JinJ Wade Harper
Feb 10, 2004·Molecular Biology of the Cell·Yoshinori Katsuragi, Noriyuki Sagata
Nov 19, 2004·Nature·Michael B Kastan, Jiri Bartek
Dec 9, 2004·Journal of Cell Science·Stephen DunawayNancy C Walworth
Feb 15, 2005·The Biochemical Journal·Catriona A L Clarke, Paul R Clarke
Feb 16, 2005·Cancer Cell·Janusz PucRamon Parsons
Mar 16, 2005·Molecular and Cellular Biology·Angela M FergusonHelen Piwnica-Worms
Dec 20, 2005·Current Biology : CB·Veronique A J SmitsStephen P Jackson
Sep 28, 2006·Journal of Cell Science·Estelle SchmittChristine Dozier
Oct 4, 2006·Molecular and Cellular Biology·Van Leung-PinedaHelen Piwnica-Worms
Jun 15, 2007·Nature Reviews. Cancer·Rose BoutrosBernard Ducommun
Oct 20, 2007·Nature Protocols·Hidemasa Goto, Masaki Inagaki
Dec 13, 2007·Cell Cycle·Dipankar Ray, Hiroaki Kiyokawa
Nov 6, 2008·Biochemical and Biophysical Research Communications·Yosuke IkegamiMasaki Inagaki
Jun 2, 2009·DNA Repair·Duaa H Mohammad, Michael B Yaffe
Sep 8, 2009·Nature Cell Biology·Marina MelixetianKristian Helin

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Citations

Oct 4, 2011·Oncogene·R H Medema, L Macůrek
Feb 24, 2012·Molecular Biology of the Cell·Ping LiMasaki Inagaki
Sep 7, 2012·PLoS Pathogens·Mengxi JiangMichael J Imperiale
Jun 15, 2014·Apoptosis : an International Journal on Programmed Cell Death·Feng-Ze WangBao-Liang Sun
Mar 20, 2013·Cellular and Molecular Life Sciences : CMLS·Mallikarjun PatilZheng Dong
Aug 22, 2015·Biomolecules·Jan Benada, Libor Macurek
Apr 24, 2015·Molecular Biology of the Cell·Douglas R Mackay, Katharine S Ullman
Nov 12, 2014·International Journal of Oncology·De-Yu ChenWen-Qing Qi
Apr 12, 2017·FEBS Open Bio·Mark C de GooijerOlaf van Tellingen
Mar 10, 2015·Cell Structure and Function·Hidemasa GotoMasaki Inagaki
Mar 22, 2015·Mutation Research. Reviews in Mutation Research·Marina A González Besteiro, Vanesa Gottifredi
Oct 2, 2014·Nature Communications·Kousuke KasaharaMasaki Inagaki
Jan 13, 2019·Journal of Cell Science·Hidemasa GotoAkira Mizoguchi
Apr 25, 2013·International Journal of Cancer. Journal International Du Cancer·Youwei Zhang, Tony Hunter
Aug 14, 2018·Oncotarget·Bor-Jang HwangA-Lien Lu
Aug 2, 2019·The Journal of Cell Biology·Jone MichelenaMatthias Altmeyer
Dec 15, 2010·The Journal of Biological Chemistry·Jun-Ho Lee, Hua Lu
Jul 29, 2015·The FEBS Journal·Veronique A J Smits, David A Gillespie
May 2, 2020·Signal Transduction and Targeted Therapy·Rui-Xue Huang, Ping-Kun Zhou
Jan 28, 2021·Molecular Cell·Camilla FrattiniAngelos Constantinou
Jun 2, 2011·Journal of Cell Science·Makoto MatsuyamaMasaki Inagaki
Nov 14, 2021·Oncogene·Xiaoning WuValentine M Macaulay

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