PMID: 6477874May 22, 1984

14N NMR of lipid bilayers: effects of ions and anesthetics

Biochemistry
D J SiminovitchK R Jeffrey

Abstract

The interaction of divalent and trivalent metal cations, ferricyanide, a lipophilic ion (tetraphenylborate), and a local anesthetic (tetracaine) with the phosphocholine head group of egg lecithin was investigated by using wide-line 14N and 31P NMR. Measurements of the 14N quadrupolar splittings in the presence of a variety of perturbing agents demonstrated that the 14N NMR technique can be used to directly monitor ion or anesthetic binding. The 14N quadrupolar splitting (delta nu Q) is a measure of the order parameter of the C beta-N bond segment, and changes in delta nu Q as large as 3.5 kHz were observed. Moreover, a comparison of the changes in the quadrupolar splittings induced by the binding of ions or anesthetics provided a sensitive method of discriminating between these perturbing agents in their ability to alter the orientational order of the C beta-N bond segment of the phosphocholine moiety. Without exception, addition of metal ions or anesthetics always resulted in a decrease of the 14N delta nu Q. This reduction reflects a change in the average orientation or degree of motional averaging at the C beta-N bond segment position. In the case of metal ion binding, the strength of the interaction increased with the charg...Continue Reading

References

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Citations

Oct 27, 2011·Progress in Nuclear Magnetic Resonance Spectroscopy·Luke A O'Dell
Nov 7, 2008·Journal of Magnetic Resonance·Chunqi QianZhehong Gan
Jun 26, 2007·Chemistry & Biodiversity·Christiane HeuberKlaus Müller
Dec 8, 2010·Biochimica Et Biophysica Acta·Avigdor Leftin, Michael F Brown
Sep 22, 2012·Biophysical Journal·Jacques P F DouxJ Antoinette Killian
May 11, 2017·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Xiuli Xia, Yuanzhi Shao
May 29, 2018·The European Physical Journal. E, Soft Matter·Joanna Kotyńska, Zbigniew A Figaszewski

Related Concepts

Anions
Cations
Lipid Bilayers
Metals, Rare Earth
In Vivo NMR Spectroscopy
Phosphatidylcholines
Pontocaine

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