15-Deoxy-delta 12-14-prostaglandin-J2 induces hypertrophy and loss of contractility in human testicular peritubular cells: implications for human male fertility

Endocrinology
Christoph SchellArtur Mayerhofer

Abstract

The wall of the seminiferous tubules contains contractile smooth-muscle-like peritubular cells, thought to be important for sperm transport. Impaired spermatogenesis in men typically involves remodeling of this wall, and we now found that smooth muscle cell (SMC) markers, namely myosin heavy chain (MYH11) and smooth muscle actin (SMA) are often lost or diminished in peritubular cells of testes of men with impaired spermatogenesis. This suggests reduced contractility of the peritubular wall, which may contribute to sub- or infertility. In these cases, testicular expression of cyclooxygenase-2 (COX-2) implies formation of prostaglandins (PGs). When screening different PGs for their ability to target human testicular peritubular cells (HTPCs), only a PG metabolite, 15-deoxy-Delta(12-14)-prostaglandin-J2 (15dPGJ2), was effective. In primary cultures of HTPCs, 15dPGJ2 increased cell size in a reversible manner. Importantly, 15dPGJ2 treatment resulted in a loss of typical differentiation markers for SMCs, namely MYH11, calponin, and SMA, whereas fibroblast markers were unchanged. Collagen gel contraction assays revealed that this loss correlates with a reduced ability to contract. Experiments with an antagonist (bisphenol A diglycidy...Continue Reading

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Citations

Feb 23, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Artur Mayerhofer
Oct 2, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Moïra RossittoBrigitte Boizet-Bonhoure
Jun 7, 2012·Spermatogenesis·Mirja NurmioKirsi Jahnukainen
Dec 17, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Mónica B FrungieriMaría E Matzkin
May 5, 2016·Seminars in Cell & Developmental Biology·Filipe Tenorio Lira NetoMarc Goldstein
Jun 1, 2017·International Journal of Molecular Sciences·Mónica Beatriz FrungieriSoledad Paola Rossi
May 10, 2018·Andrologia·M B FrungieriM E Matzkin
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Jun 15, 2011·Physiology·Ye Chun RuanHsiao Chang Chan
Apr 2, 2014·The Journal of Clinical Endocrinology and Metabolism·Luise LandrehArtur Mayerhofer
Oct 23, 2019·Scientific Reports·Nina SchmidArtur Mayerhofer
Mar 7, 2021·International Journal of Molecular Sciences·Nina SchmidArtur Mayerhofer
Apr 17, 2018·Toxicology and Applied Pharmacology·Amélie R Tremblay, Geraldine Delbes

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