15-Deoxy-Delta12,14-prostaglandin J2 and thiazolidinediones transactivate epidermal growth factor and platelet-derived growth factor receptors in vascular smooth muscle cells

Biochemical and Biophysical Research Communications
T IchikiA Takeshita

Abstract

Proliferation of vascular smooth muscle cells (VSMCs) is induced by various mitogens through activation of extracellular signal-regulated protein kinase (ERK) pathway. We recently reported that peroxisome proliferator-activated receptor (PPAR)gamma activators such as 15-deoxy-Delta12,14-prostaglandin J2 (15-d-PGJ2) and thiazolidinediones (TZDs) activated MEK/ERK pathway through phosphatidylinositol 3-kinase (PI3-K) and induced proliferation of VSMCs. However, the precise mechanisms of PPARgamma activators-induced activation of PI3-K/ERK pathway have not been determined. We examined whether transactivation of growth factor receptor is involved in this process. Stimulation of VSMCs with 15-d-PGJ2 or TZDs for 15 min induced phosphorylation of ERK1/2 and Akt. 15-d-PGJ2- or TZDs-induced phosphorylation of ERK1/2 and Akt was inhibited by AG1478, an inhibitor of epidermal growth factor receptor (EGF-R) as well as AG1295, an inhibitor of platelet derived growth factor receptor (PDGF-R). 15-d-PGJ2-induced phosphorylation of both EGF-R and PDGF-R. GM6001, a matrix metalloproteinase inhibitor, and PP2, a Src family protein kinase inhibitor, suppressed 15-d-PGJ2- and TZDs-induced phosphorylation of EGF-R and PDGFbeta-R as well as activatio...Continue Reading

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Citations

Sep 6, 2011·Basic Research in Cardiology·Yumei YeYochai Birnbaum
Aug 2, 2007·Expert Opinion on Therapeutic Targets·Efstathia PapageorgiouMichael Koutsilieris
Mar 13, 2015·Frontiers in Immunology·Katie L LannanRichard P Phipps
Sep 30, 2005·Toxicology·Seong-Ho KohSeung Hyun Kim
Mar 21, 2007·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yajun ZhouAnping Chen
Apr 11, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Yumei YeYochai Birnbaum
Jul 14, 2017·Canadian Journal of Physiology and Pharmacology·Ekhtear Hossain, Madhu B Anand-Srivastava
Jul 23, 2014·Molecular and Cellular Biochemistry·Katarina SmiljanicEsma R Isenovic

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