15-Deoxy-Delta12,14-prostaglandin J2 regulates mesangial cell proliferation and death

Kidney International
Brad H RovinTodd Hilbelink

Abstract

Proliferation of intrinsic glomerular cells is a common response to renal injury. Acutely, proliferation may be beneficial, but sustained glomerular hypercellularity after injury is associated with progressive renal failure. To identify endogenous factors that may be responsible for regulating glomerular cell number, the effects of J-series cyclopentenone prostaglandins (PGs) on human glomerular mesangial cell proliferation and death were examined. Human mesangial cells were grown in the presence or absence of PGJ2 or its metabolite 15-Deoxy-Delta12,14-PGJ2 (15dPGJ2). The number of viable cells was measured by the reduction of the tetrazolium MTS to a colored formazan product. Apoptosis was assessed by caspase-3 activation and DNA fragmentation. PGJ2 at concentrations up to 10 micromol/L caused mesangial proliferation. 15dPGJ2 also caused mesangial proliferation at low concentrations (< or =2.5 micromol/L), but induced mesangial cell death at higher concentrations (>5 micromol/L). Cell death occurred in part through apoptosis, measured as an increase in caspase-3 activity and DNA fragmentation in 15dPGJ2-treated cells. Cell death was associated with a decline in baseline phosphorylation of the survival factor Akt and increased ...Continue Reading

References

Sep 1, 1992·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M Fukushima
Sep 1, 1990·Prostaglandins, Leukotrienes, and Essential Fatty Acids·E A Lianos
Mar 1, 1986·Journal of Periodontal Research·S OffenbacherT E Van Dyke
Jul 25, 1997·The Journal of Biological Chemistry·L FajasJ Auwerx
Jun 17, 1999·Nature Medicine·D W GilroyD A Willoughby
Dec 28, 1999·Kidney International·H O SchöcklmannR B Sterzel
Dec 28, 1999·Kidney International·J Savill
Jan 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C M ReillyG S Gilkeson
Feb 2, 2000·Current Opinion in Nephrology and Hypertension·M D Breyer, R M Breyer
Mar 1, 2000·British Journal of Pharmacology·D Bishop-Bailey
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·D S StrausC K Glass
May 17, 2000·Journal of Cardiovascular Pharmacology·S GoetzeR E Law
Aug 11, 2000·Prostaglandins & Other Lipid Mediators·C E ClayF H Chilton
Nov 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S HortelanoL Boscá
Dec 20, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J PadillaR P Phipps
Feb 17, 2001·Biochemical and Biophysical Research Communications·W A WilmerB H Rovin
Mar 30, 2001·The Journal of Biological Chemistry·M KondoK Uchida
Jun 16, 2001·American Journal of Physiology. Renal Physiology·R C Harris, M D Breyer
Jun 16, 2001·The Journal of Biological Chemistry·D TangA J Ingram
Jul 20, 2001·Journal of the American Society of Nephrology : JASN·Brad H RovinAnna Cosio
Aug 29, 2001·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·C E ClayF H Chilton

❮ Previous
Next ❯

Citations

Dec 20, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Rachel J A HelliwellMurray D Mitchell
Apr 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jose Luis OlivaJosé M Rojas
Apr 26, 2007·Kidney International·T KanjanabuchA B Fogo
Jul 6, 2006·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Mohammad NewazAdebayo Oyekan
Nov 4, 2004·Chemico-biological Interactions·Patricia E GaneyRobert A Roth
Oct 16, 2003·The Journal of Biological Chemistry·Antonio E RusiñolMichael S Sinensky
Apr 24, 2003·The Journal of Biological Chemistry·Takahiro ShibataKoji Uchida
Apr 7, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Delphine GrasCaroline Bonnans
Jun 17, 2020·Current Diabetes Reports·Christopher D Wiley

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.