15-Lipoxygenase-1 transcriptional silencing by DNA methyltransferase-1 independently of DNA methylation

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Xiangsheng ZuoImad Shureiqi

Abstract

Methylation of promoter DNA contributes to transcriptional silencing of various tumor-suppressor genes in cancer. Transcriptional silencing of 15-lipoxygenase-1 (15-LOX-1) promotes tumorigenesis. Methylation of 15-LOX-1 promoter DNA occurs in some cancers, but its mechanistic role in 15-LOX-1 transcriptional silencing is unclear. We examined the mechanistic role of 15-LOX-1 promoter DNA methylation in 15-LOX-1 transcriptional regulation in human colorectal cancers. 15-LOX-1 promoter methylation occurred in colorectal cancer cells in vitro, in 36% of tumor tissue samples of colorectal cancer patients, and in virtually no normal colonic mucosa samples of 50 human subjects with no history of colorectal cancer or polyps. 15-LOX-1 promoter DNA methylation levels, however, did not correlate with 15-LOX-1 expression levels (Spearman's r=0.21; P=0.38). We employed siRNA knockdown and genetic disruption models of DNA methyltransferases (DNMTs) to study the effects of this methylation on 15-LOX-1 expression in colon cancer cells. 15-LOX-1 promoter demethylation was insufficient to reestablish 15-LOX-1 expression. 15-LOX-1 transcription was activated by the histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA) only after DN...Continue Reading

References

Oct 15, 1991·Biochemical and Biophysical Research Communications·A N BaerF A Green
Nov 1, 1990·The New England Journal of Medicine·A EkbomH O Adami
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·A R BrashM S Chang
Feb 13, 1999·Nature Genetics·P A Jones, P W Laird
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·K A MungerK F Badr
Dec 30, 1999·Nature Genetics·F FuksT Kouzarides
Sep 12, 2001·The Journal of Biological Chemistry·D R HodgeW L Farrar
Apr 5, 2002·Nature·Ina RheeBert Vogelstein
Apr 19, 2003·Science·François GaudetRudolf Jaenisch
Apr 25, 2003·Nucleic Acids Research·François FuksTony Kouzarides
Aug 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Imad ShureiqiScott M Lippman
Nov 5, 2003·Nature Immunology·Karen W MakarChristopher B Wilson
Dec 4, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Jennifer B NixonThomas E Eling
Dec 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Charles N SerhanThomas E Van Dyke
Jun 15, 2004·Experimental Cell Research·Cheng LiuHans-Erik Claesson
Mar 16, 2005·Cancer Cell·Frances BalkwillAlberto Mantovani
Jun 3, 2005·Cancer Research·David R HodgeWilliam L Farrar
Oct 12, 2005·The Journal of Biological Chemistry·Amiram ArielCharles N Serhan
Dec 17, 2005·Nature·Emmanuelle ViréFrançois Fuks
Mar 25, 2006·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Wen G JiangRobert E Mansel
May 9, 2006·Progress in Lipid Research·Hartmut Kühn, Valerie B O'Donnell
May 19, 2006·Cancer Research·Jingmin ShuJean-Pierre J Issa
Sep 12, 2006·Proceedings of the National Academy of Sciences of the United States of America·Gerda EggerGangning Liang
Oct 5, 2006·Proceedings of the National Academy of Sciences of the United States of America·Marika MassaroRaffaele De Caterina
Mar 6, 2007·Trends in Immunology·Amiram Ariel, Charles N Serhan
Mar 21, 2007·Molecular and Cellular Biology·Marc Damelin, Timothy H Bestor
Nov 22, 2007·Neoplasia : an International Journal for Oncology Research·René HennigThomas E Adrian

❮ Previous
Next ❯

Citations

Oct 12, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Masoumeh Tavakoli-YarakiMajid Sirati-Sabet
Aug 13, 2013·Prostaglandins & Other Lipid Mediators·Adi J Klil-Drori, Amiram Ariel
Sep 19, 2008·The Journal of Biological Chemistry·Xiangsheng ZuoImad Shureiqi
Jun 24, 2010·Cancer Prevention Research·Imad ShureiqiScott M Lippman
Nov 1, 2011·Cancer Metastasis Reviews·Sun Il LeeImad Shureiqi
Sep 18, 2012·Molecular Aspects of Medicine·Silvia UdaliSimonetta Friso
Dec 18, 2009·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·A Comba, María Eugenia Pasqualini
Aug 1, 2018·Cell Proliferation·Melis ÇolakoğluSreeparna Banerjee
Nov 12, 2015·World Journal of Gastroenterology : WJG·Sinem Tuncer, Sreeparna Banerjee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Epigenetics & Methyl-CpG (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. Here is the latest research on cancer epigenetics and methyl-CpG binding proteins including ZBTB38.

Cancer Epigenetics

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.

Cell Signaling & Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. This feed covers the latest research on signaling and epigenetics in cell growth and cancer.

Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.

Cancer Epigenetics & Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.

Cancer Epigenetics and Senescence (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may be involved in regulating senescence in cancer cells. This feed captures the latest research on cancer epigenetics and senescence.

Related Papers

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Imad ShureiqiScott M Lippman
Molecular Therapy : the Journal of the American Society of Gene Therapy
Yuanqing WuImad Shureiqi
International Journal of Cancer. Journal International Du Cancer
Hong ZhuT E Eling
© 2021 Meta ULC. All rights reserved