15Alpha-hydroxyprogesterone in male sea lampreys, Petromyzon marinus L

Steroids
Mara B BryanWeiming Li

Abstract

There is growing evidence that sea lampreys, Petromyzon marinus L., produce gonadal steroids differing from those of other vertebrates by possessing an additional hydroxyl group at the C15 position. Here we demonstrate that sea lamprey testes produce 15alpha-hydroxyprogesterone (15alpha-P) in vitro when incubated with tritiated progesterone, that 15alpha-P is present in the plasma of sea lampreys, and that plasma concentrations of immunoreactive (ir) 15alpha-P rise dramatically in response to injections of gonadotropin-releasing hormone (GnRH). The identity of the tritiated 15alpha-P produced in vitro was confirmed by co-elution with standard 15alpha-P on high performance liquid chromatography, co-elution with standard and acetylated 15alpha-P on thin layer chromatography, and specific binding to antibodies raised against standard 15alpha-P. The in vitro conversion was used to produce tritiated 15alpha-P label for a radioimmunoassay (RIA), which is able to detect 15alpha-P in amounts as low as 2 pg per tube. The RIA has been used to measure the plasma concentrations of 15alpha-P in males given two serial injections, 24 h apart, of either lamprey GnRH I or GnRH III (50, 100, or 200 microg/kg) or saline control, with plasma being...Continue Reading

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Jul 10, 2003·General and Comparative Endocrinology·Mara B BryanWeiming Li
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Citations

Nov 30, 2013·General and Comparative Endocrinology·Brent W RobertsDavid A Close
Jun 27, 2009·PloS One·Michael E BakerCharlie Chandsawangbhuwana
Jan 8, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Huiyong WangWeiming Li
Sep 29, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Matthew G MesaStacia A Sower
May 13, 2008·Biochemical and Biophysical Research Communications·Michael E Baker, Charlie Chandsawangbhuwana
Jan 16, 2008·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Takanobu MizutaKaoru Kubokawa
Dec 20, 2005·General and Comparative Endocrinology·Mara B BryanWeiming Li
May 7, 2013·General and Comparative Endocrinology·Yu-Wen Chung-DavidsonWeiming Li
Jun 29, 2007·Biology of Reproduction·Mara B BryanWeiming Li
Oct 13, 2007·Steroids·Mara B BryanWeiming Li

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