15N-1H Residual dipolar coupling analysis of native and alkaline-K79A Saccharomyces cerevisiae cytochrome c

Biophysical Journal
Michael AssfalgHarry B Gray

Abstract

Residual dipolar couplings (RDCs) and pseudocontact shifts are experimentally accessible properties in nuclear magnetic resonance that are related to structural parameters and to the magnetic susceptibility anisotropy. We have determined RDCs due to field-induced orientation of oxidized-K79A and reduced cytochrome c at pH 7.0 and oxidized-K79A cytochrome c at pH 11.1 through measurements of amide (15)N-(1)H (1)J couplings at 800 and 500 MHz. The pH 7.0 RDCs for Fe(III)- and Fe(II)-cytochrome c together with available nuclear Overhauser effects were used to recalculate solution structures that were consistent with both sets of constraints. Molecular magnetic susceptibility anisotropy values were calculated for both redox states of the protein. By subtracting the residual dipolar couplings (RDCs) of the reduced form from those of the oxidized form measured at the same magnetic field (800 MHz), we found the RDC contribution of the paramagnetic metal ion in the oxidized protein. The magnetic susceptibility anisotropy, which was calculated from the structure, was found to be the same as that of the paramagnetic metal ion obtained independently from pseudocontact shifts, thereby indicating that the elements of secondary structure eit...Continue Reading

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Mar 6, 2003·Journal of the American Chemical Society·Michael AssfalgHarry B Gray

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Citations

Aug 10, 2011·Journal of Biomolecular NMR·Soumyasri DasguptaMarcellus Ubbink
Feb 6, 2004·Journal of Molecular Biology·Ivano BertiniGérard Simonneaux
Mar 26, 2004·Journal of Magnetic Resonance·Homayoun Valafar, James H Prestegard
Oct 9, 2013·BMC Bioinformatics·Chris SchmidtHomayoun Valafar
Oct 21, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Giovanni La PennaLucia Banci
Nov 23, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Natasa TomáskováErik Sedlák
Aug 27, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Arjang FahimHomayoun Valafar
Mar 18, 2016·Journal of Chemical Theory and Computation·Casey Anne ColeHomayoun Valafar
Feb 9, 2008·Journal of Magnetic Resonance·Michael BrysonHomayoun Valafar
Jan 29, 2014·Journal of Bioinformatics and Computational Biology·Rishi MukhopadhyayHomayoun Valafar
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Mar 2, 2017·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Shun HirotaSatoshi Nagao
May 10, 2007·Biochemistry·Michael AssfalgPaola Turano
Dec 23, 2017·Biochemistry·Dillon B NyeJuliette T J Lecomte

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