15N-NMR characterization of His residues in and around the active site of FeSOD.

Biochimica Et Biophysica Acta
Anne-Frances MillerSurekha Vathyam

Abstract

We have exploited (15)N-NMR to observe histidine (His) side chains in and around the active site of Fe-containing superoxide dismutase (FeSOD). In the oxidized state, we observe all the non-ligand His side chains and in the reduced state we can account for all the signals in the imidazole spectral region in terms of the non-ligand His', paramagnetically displaced signals from two backbone amides, and the side chain of glutamine 69 (Gln69). We also observe signals from the His' that ligate Fe(II). These confirm that neither the Q69H nor the Q69E mutation strongly affects the Fe(II) electronic structure, despite the 250 mV and >660 mV increases in E(m) they produce, respectively. In the Q69H mutant, we observe two new signals attributable to the His introduced into the active site in place of Gln69. One corresponds to a protonated N and the other is strongly paramagnetically shifted, to 500 ppm. The strong paramagnetic effects support the existence of an H-bond between His69 and the solvent molecule coordinated to Fe(II), as proposed based on crystallography. Based on previous information that His69 is neutral, we infer that the shifted N is not protonated. Therefore, we propose that this N represents a site of H-bond acceptance ...Continue Reading

References

Jan 1, 1991·Free Radical Research Communications·W C StallingsM L Ludwig
May 15, 1981·Biochemical and Biophysical Research Communications·J A FeeE M Fielden
Sep 17, 1999·The Journal of Biological Chemistry·C A RamiloD N Silverman
Apr 4, 2002·Journal of the American Chemical Society·Emine YikilmazAnne-Frances Miller
Apr 4, 2002·Journal of the American Chemical Society·Juan XieThomas C Brunold
Jan 23, 2003·Journal of Inorganic Biochemistry·Anne-Frances MillerCarrie K Vance
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·John T Groves
Mar 2, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Kevin D KoehntopLawrence Que
Dec 27, 2005·The Journal of Physical Chemistry. B·Isabel A AbreuDiane E Cabelli
Jul 14, 2007·Journal of the American Chemical Society·Emine YikilmazAnne-Frances Miller
Jan 1, 1997·Methods in Enzymology·E A Merritt, D J Bacon

❮ Previous
Next ❯

Citations

Nov 15, 2011·FEBS Letters·Anne-Frances Miller
Jul 25, 2015·Nature Communications·Poonam SrivastavaJared C Lewis
Dec 29, 2017·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Muhamed AminMohamed H Alkordi
Apr 2, 2014·Chemical Reviews·Yuewei ShengJoan Selverstone Valentine

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.