1,6-Diphenyl-1,3,5-hexatriene (DPH) as a Novel Matrix for MALDI MS Imaging of Fatty Acids, Phospholipids, and Sulfatides in Brain Tissues

Analytical Chemistry
Hanadi IbrahimKen K-C Yeung

Abstract

1,6-Diphenyl-1,3,5-hexatriene (DPH) is a commonly used fluorescence probe for studying cell membrane-lipids due to its affinity toward the acyl chains in the phospholipid bilayers. In this work, we investigated its use in matrix-assisted laser desorption/ionization (MALDI) as a new matrix for mass spectrometry imaging (MSI) of mouse and rat brain tissue. DPH exhibits very minimal matrix-induced background signals for the analysis of small molecules (below m/z of 1000). In the negative ion mode, DPH permits the highly sensitive detection of small fatty acids (m/z 200-350) as well as a variety of large lipids up to m/z of 1000, including lyso-phospholipid, phosphatidic acid (PA), phosphoethanolamine (PE), phosphatidylserine (PS), phosphatidylglycerol (PG), phosphatidylinositol (PI), and sulfatides (ST). The analytes were mostly detected as the deprotonated ion [M - H]-. Our results also demonstrate that sublimated DPH is stable for at least 24 h under the vacuum of our MALDI mass spectrometer. The ability to apply DPH via sublimation coupled with its low volatility allows us to perform tissue imaging of the above analytes at high spatial resolution. The degree of lipid fragmentation was determined experimentally at varying laser ...Continue Reading

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Citations

Nov 6, 2018·The Journal of Comparative Neurology·Guang Xu, Jianjun Li
Mar 9, 2019·Annual Review of Analytical Chemistry·Ian S GilmoreCornelius L Pieterse
Aug 15, 2019·Journal of Mass Spectrometry : JMS·Ethan YangPierre Chaurand
Jul 28, 2020·Trends in Analytical Chemistry : TRAC·Jianing Wang, Xianlin Han
Jan 29, 2021·Expert Review of Proteomics·Al MamunMitsutoshi Setou
Nov 21, 2020·Analytical and Bioanalytical Chemistry·Qiuqin ZhouCarsten Hopf
Mar 18, 2021·Journal of the American Society for Mass Spectrometry·Eliise TammekiviIvo Leito
Apr 28, 2021·Journal of the American Society for Mass Spectrometry·Jing YuYiping Du
Dec 24, 2021·Journal of the American Society for Mass Spectrometry·Huizhi LiT-W Dominic Chan

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