16,16-Dimethyl Prostaglandin E2 modulation of endothelial monolayer paracellular barrier function

Journal of Cellular Physiology
T Y Ma, A Pedram

Abstract

Prostaglandins prevent gastrointestinal mucosal injury and promote healing following mucosal injury by various noxious agents. Preservation or repair of microvascular function appears to be crucial in these processes. The processes involved in prostaglandin-mediated repair and preservation of endothelial function are unclear. In the present study, we investigated the role of prostaglandins on endothelial paracellular barrier function using the filter-grown bovine aortic endothelial cell monolayers. Endothelial paracellular barrier function as assessed using a paracellular marker, mannitol. Prostaglandin analogs 16,16-dimethyl prostaglandin E2 (DMPGE2) and prostaglandin I2 (PGI2) caused an enhancement of endothelial monolayer paracellular barrier function as evidenced by a dose-dependent decrease in endothelial paracellular permeability. DMPGE2 induced enhancement of endothelial paracellular barrier function correlated directly with increasing intracellular cAMP levels. Agents which increase intracellular cAMP levels at different stages of cAMP amplification cascade including phosphodiesterase inhibitor (3-isobutyl-1 methylxanthine [IBMX]), membrane permeable cAMP (8-bromo cAMP), and adenylate cyclase activators (isoproterenol a...Continue Reading

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Citations

Jun 27, 2000·Prostaglandins & Other Lipid Mediators·B Mohajer, T Y Ma
Sep 2, 1999·Critical Care Medicine·P E Marik, J Iglesias
May 18, 2005·Microvascular Research·Natalia V BogatchevaAlexander D Verin
Apr 6, 2006·Journal of Lipid Research·Raquel Martín-VenegasJuan José Moreno
Aug 15, 2001·American Journal of Physiology. Cell Physiology·S J SawyerF M Pavalko

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