PMID: 1987160Jan 1, 1991Paper

16S ribosomal DNA amplification for phylogenetic study

Journal of Bacteriology
W G WeisburgD J Lane

Abstract

A set of oligonucleotide primers capable of initiating enzymatic amplification (polymerase chain reaction) on a phylogenetically and taxonomically wide range of bacteria is described along with methods for their use and examples. One pair of primers is capable of amplifying nearly full-length 16S ribosomal DNA (rDNA) from many bacterial genera; the additional primers are useful for various exceptional sequences. Methods for purification of amplified material, direct sequencing, cloning, sequencing, and transcription are outlined. An obligate intracellular parasite of bovine erythrocytes, Anaplasma marginale, is used as an example; its 16S rDNA was amplified, cloned, sequenced, and phylogenetically placed. Anaplasmas are related to the genera Rickettsia and Ehrlichia. In addition, 16S rDNAs from several species were readily amplified from material found in lyophilized ampoules from the American Type Culture Collection. By use of this method, the phylogenetic study of extremely fastidious or highly pathogenic bacterial species can be carried out without the need to culture them. In theory, any gene segment for which polymerase chain reaction primer design is possible can be derived from a readily obtainable lyophilized bacterial ...Continue Reading

References

Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J BrosiusH F Noller
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Oct 1, 1985·Journal of Bacteriology·W G WeisburgC R Woese
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D J LaneN R Pace
Jun 1, 1987·Microbiological Reviews·C R Woese
Aug 1, 1989·Journal of Bacteriology·W G WeisburgC R Woese
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E WeissY H Kang
Jul 11, 1988·Nucleic Acids Research·R RossauH Van Heuverswyn
Jan 1, 1985·Progress in Nucleic Acid Research and Molecular Biology·R GutellH F Noller
Nov 1, 1985·Science·W G WeisburgE Weiss
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M D BigginG F Hong
Jul 25, 1980·Science·G E FoxC R Woese

Citations

May 23, 1991·The New England Journal of Medicine
Aug 1, 1993·FEMS Microbiology Letters·D RalphM McClelland
Jan 1, 1996·Annual Review of Genetics·W M Huang
Jul 1, 1997·FEMS Microbiology Reviews·D L BalkwillD R Boone
Mar 26, 1998·FEMS Microbiology Letters·H Neimark, K M Kocan
May 8, 1999·The American Journal of Clinical Nutrition·R I MackieH R Gaskins
Oct 19, 1999·FEMS Microbiology Ecology·N S ReyesP A Sobecky
Feb 26, 2000·FEMS Microbiology Letters·C McDevittA G McEwan
Nov 30, 2000·Current Infectious Disease Reports·P E Fournier, D Raoult
Sep 6, 2000·Proceedings. Biological Sciences·J H Werren, D M Windsor
Apr 5, 2001·FEMS Microbiology Letters·A CasanovaM Jashés
Aug 16, 2001·The Journal of Veterinary Medical Science·H InokumaP Brouqui
Feb 19, 2002·Journal of Applied Microbiology·D LindsayA von Holy
Oct 30, 2001·FEMS Microbiology Letters·M Horn, M Wagner
Jun 14, 2002·Journal of Medical Entomology·Timothy J KurttiJames H Oliver
Jul 18, 2002·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·B KokotovicP Ahrens
Jul 16, 2003·Annals of the New York Academy of Sciences·Oleg MediannikovHiroshi Suzuki
Sep 10, 2003·Journal of Fish Diseases·C CepedaY Santos
Oct 11, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Karen W PostStephen J Billington
Apr 28, 2004·Emerging Infectious Diseases·Gilbert GreubDidier Raoult
Jun 3, 2004·Nature Reviews. Immunology·Andrew J Macpherson, Nicola L Harris
Nov 19, 2004·Archives of Microbiology·Mette M SvenningUlla Rasmussen
Feb 24, 2005·Antonie van Leeuwenhoek·Naomi F MontalvoRussell T Hill

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