16S ribosomal RNA sequencing reveals a modulation of intestinal microbiome and immune response by dietary L-theanine supplementation in broiler chickens

Poultry Science
Muhammad SaeedSun Chao

Abstract

Despite the availability of abundant literature on green tea, studies on the use of L-theanine (an amino acid found only in green tea) as a feed additive in poultry especially broiler are limited. So, this study was conducted to explore the effects of L-theanine on the intestinal microbiome and immune response in a broiler. A total of 400-d-old chicks were randomly divided into four treatment groups (A, B, C, and D) using a complete randomized design. Treatments were as follows: A, control (basal diet); B, basal diet + 100 mg L-theanine/kg diet; C, basal diet + 200 mg L-theanine/kg diet; and D, basal diet + 300 mg L-theanine/kg diet. Mucosal samples from ileum and jejunum of broiler chicken were extracted at 21 and 42 d of age. Extraction of genomic DNA was followed by amplification of V3 and V4 hypervariable regions of 16S ribosomal RNA. After Illumina sequencing, results revealed that treatment with L-theanine significantly increased the population of Lactobacillus in ileum and jejunum as compared to a control group, but the higher population was observed in jejunum at both 21 and 42 d of age. The overall diversity of the jejunum microbiome in the treatment group was significantly lower than that of the ileum and control grou...Continue Reading

References

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Citations

Oct 9, 2019·Journal of Animal Physiology and Animal Nutrition·Mahmoud AlagawanyKuldeep Dhama
Nov 5, 2020·Poultry Science·Muhammad SaeedSun Chao

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Methods Mentioned

BETA
Illumina sequencing
PCR

Software Mentioned

COPE ( Connecting Overlapped Pair - End )
Mothur
RDP classifier
QIIME
Mothur package
R

Related Concepts