16S rRNA molecular profiling of heavy metal tolerant bacterial communities isolated from soil contaminated by electronic waste.

Folia Microbiologica
Pankaj KumarRajesh Kumar

Abstract

Electronic waste is an evolving source of harmful pollutants in our surrounding environments and considered to be perilous as it contains toxic metals such as chromium, cadmium, lead, mercury, zinc, and nickel in huge quantities. Heavy metals are harmful contaminants and accumulated in the environment due to various anthropogenic activities. The present study was conducted to isolate and characterize different heavy metal tolerant bacterial species, based on molecular techniques from soil contaminated by electronic waste. The contaminated soil samples were analyzed for various physicochemical properties such as pH, electrical conductivity, soil moisture, water holding capacity, organic carbon, organic matter, available phosphorus, total nitrogen, and potassium using standard procedures. The soil samples were found to contain a higher amount of different heavy metals such as copper, chromium, lead, iron, cadmium, and nickel. Serial dilution and spread plate techniques have been used for bacterial isolation. The identification and molecular characterization of isolated bacterial species were done by biochemical tests and 16S rRNA gene sequencing technique. The 16S rRNA sequencing analysis confirmed the presence of different bacte...Continue Reading

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Citations

Aug 13, 2021·Environmental Science and Pollution Research International·Pankaj Kumar, Madhusudan Hiraman Fulekar

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Datasets Mentioned

BETA
MH714557

Methods Mentioned

BETA
electrophoresis

Software Mentioned

MEGA
Basic Local Alignment Search Tool ( BLAST )
BLAST
Origin
Clustal W

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