PMID: 2117459Jun 1, 1990Paper

16S rRNA sequences reveal uncultured inhabitants of a well-studied thermal community

FEMS Microbiology Reviews
D M WardMary M Bateson

Abstract

Molecular methods are beginning to reveal inhabitants of natural microbial communities which have never before been cultured. Our approach involves selective cloning of naturally occurring 16S rRNA sequences as cDNA, and comparison of these sequences to a database which includes 16S rRNA sequences of isolated community members. We provide here an overview of the method and its potential for community analysis. A 16S rRNA sequence retrieved from the well-studied hot spring cyanobacterial mat in Octopus Spring (Yellowstone National Park) is shown as an example of one contributed by an uncultured member of the community.

References

Jun 1, 1985·Applied and Environmental Microbiology·D A StahlN R Pace
Oct 1, 1985·Journal of Bacteriology·W G WeisburgC R Woese
Jan 1, 1986·Annual Review of Microbiology·G J OlsenD A Stahl
Jan 1, 1988·Methods in Enzymology·D J LaneN R Pace
Aug 1, 1988·Journal of Bacteriology·S J GiovannoniN R Pace
Jan 1, 1988·Methods in Enzymology·G J Olsen
May 1, 1988·Applied and Environmental Microbiology·D A StahlL Montgomery
Jan 1, 1987·Cold Spring Harbor Symposia on Quantitative Biology·G J Olsen
Aug 1, 1986·Journal of Bacteriology·W G WeisburgC R Woese
Jan 1, 1983·Molecular & General Genetics : MGG·N Tomioka, M Sugiura
Jul 1, 1980·Journal of Bacteriology·J G ZeikusP W Hegge
Jan 1, 1985·Systematic and Applied Microbiology·C R WoeseG E Fox
Jan 1, 1986·Systematic and Applied Microbiology·C R Woese, G J Olsen
Jan 1, 1986·Systematic and Applied Microbiology·T J McGillG E Fox
Jan 1, 1987·Systematic and Applied Microbiology·L Achenbach-RichterC R Woese
Jan 1, 1987·Systematic and Applied Microbiology·H OyaizuC R Woese
Oct 1, 1977·Applied and Environmental Microbiology·W N Doemel, T D Brock
Jul 1, 1988·Applied and Environmental Microbiology·M M Bateson, D M Ward
Jul 1, 1989·Applied and Environmental Microbiology·R Weller, D M Ward

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Citations

Jan 5, 1999·Applied and Environmental Microbiology·A HiraishiY Maki
Jan 10, 2006·Microbial Ecology·Duongruitai NicomratOlli H Tuovinen
Aug 13, 2009·Antonie van Leeuwenhoek·M C PortilloJ M Gonzalez
Jan 1, 2013·The Journal of Microbiology·Sung Kyum KimHoon Kim
Aug 3, 2002·Environmental Microbiology·Sasha Shafikhani
Dec 26, 2008·Extremophiles : Life Under Extreme Conditions·M C PortilloJ M Gonzalez
Sep 22, 2009·Canadian Journal of Microbiology·Véronique PrinceCarole Beaulieu
Aug 14, 1998·Environmental Health Perspectives·T FordJ Shine
Oct 8, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Patrick J Keeling
Aug 27, 2014·Nature Reviews. Microbiology·Hilary Lappin-ScottPaul Stoodley
Apr 2, 1999·Applied and Environmental Microbiology·S M BarnsC R Kuske
May 29, 1999·Applied and Environmental Microbiology·K Zepp FalzD Hahn
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·D M WardM M Bateson
Nov 1, 1996·Applied and Environmental Microbiology·J P Gray, R P Herwig
Dec 1, 1996·Journal of Clinical Microbiology·J N KriegerR E Berger
Aug 30, 2008·Food Microbiology·Jean-Luc Jany, Georges Barbier

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