16S rRNA Sequencing Reveals Relationship Between Potent Cellulolytic Genera and Feed Efficiency in the Rumen of Bulls

Frontiers in Microbiology
Emily McGovernSinéad M Waters

Abstract

The rumen microbial population dictates the host's feed degradation capacity and subsequent nutrient supply. The rising global human population and intensifying demand for animal protein is creating environmental challenges. As a consequence, there is an increasing requirement for livestock with enhanced nutrient utilization capacity in order to more efficiently convert plant material to high quality edible muscle. In the current study, residual feed intake (RFI), a widely used and a highly accepted measure of feed efficiency in cattle, was calculated for a combination of three cohorts of Simmental bulls. All animals were managed similarly from birth and offered concentrate ad libitum in addition to 3 kg of grass silage daily during the finishing period. Solid and liquid rumen digesta samples collected at slaughter and were analyzed using amplicon sequencing targeting the 16S rRNA gene utilizing the Illumina MiSeq platform. Volatile fatty acid analysis was also conducted on the liquid digesta samples. Spearman's correlation coefficient was utilized to determine the association between RFI and bacterial and archaeal taxa and inter-taxonomic relationships. The data indicate a tendency toward an increase in butyrate (P = 0.06), wh...Continue Reading

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Citations

Jan 14, 2020·Journal of Animal Science·Harvey C FreetlyJames E Wells
Sep 29, 2020·Frontiers in Microbiology·Steven McLoughlinSinéad M Waters
Apr 1, 2021·Animal : an International Journal of Animal Bioscience·Y K ZhangW M Wang
Oct 15, 2020·Microorganisms·Gonzalo Martinez-FernandezChristopher S McSweeney

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Methods Mentioned

BETA
amplicon sequencing
PCR
Illumina sequencing

Software Mentioned

Hmisc package R Studio
FASTQC
USEARCH
BBMap
QIIME
ChimeraSlayer
PRIMER7
BBMerge
UCLUST
PERMANOVA

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