PMID: 8581875Dec 1, 1995Paper

17 beta-estradiol increases the receptor number and modulates the action of 1,25-dihydroxyvitamin D3 in human osteosarcoma-derived osteoblast-like cells

Calcified Tissue International
M IshibeJ E Puzas

Abstract

It is well known that 17 beta-estradiol (E2) and 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) have important roles in bone metabolism. This study was undertaken to examine E2 regulation of 1,25(OH)2D3 receptor (VDR) expression and the biological action of 1,25(OH)2D3 in human osteoblast-like cells. When human osteosarcoma-derived osteoblast-like cells were treated with varying concentrations of E2, the VDR levels increased by up to 100% in a dose-dependent manner. VDR levels significantly increased at 10 nM E2 and this increase plateaued at 100 nM E2. E2-dependent increase of VDR was time dependent, plateauing at 24 hours and was maintained for at least 48 hours in human osteoblast-like cells. Scatchard analysis showed that E2 increased the number of VDR (12.3 +/- 0.4 versus 26.5 +/- 0.3 fmol/mg protein; mean +/- SE of three independent experiments) rather than the Kd (0.15 +/- 0.02 versus 0.16 +/- 0.01 nM; mean +/- SE of three independent experiments). Tamoxifen (50 nM), a specific competitor with E2, completely abolished the E2-induced increase of VDR. The levels of VDR mRNA (4.5 kb) from the cells increased in a dose-dependent manner after E2 treatment. With regard to the biological effects, E2 increased by 10-25% the inhibitory e...Continue Reading

References

Mar 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·P E KeetingB L Riggs
Aug 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J M PenslerC B Langman
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T Takano-Yamamoto, G A Rodan
May 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J M PenslerA Molteni
Jan 1, 1989·Journal of Steroid Biochemistry·T K Gray
Feb 10, 1989·Cell·M Beato
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D SömjenA M Kaye
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T K GrayL M Nabell
Apr 1, 1985·British Journal of Obstetrics and Gynaecology·S M Tuck, A Williams
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M ErnstE R Froesch
Mar 1, 1985·Proceedings of the Society for Experimental Biology and Medicine·W K Ramp, R L Baker
Nov 30, 1981·Biochemical and Biophysical Research Communications·M R Walters
Mar 27, 1980·The New England Journal of Medicine·P F CocciaD M Brown
Jan 1, 1994·Archives of Orthopaedic and Trauma Surgery·M IshibeK Kaneda
Apr 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·M T EscaleiraM M Brentani

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Citations

Sep 5, 2002·Journal of Biomedical Materials Research·C H LohmannZ Schwartz
Mar 2, 2005·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·Stefano PalombaFulvio Zullo
Dec 9, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y LielB Schwartz
Apr 24, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A ShaoJ C Fleet
Dec 4, 2012·Nutrients·Clara Y Park, Connie M Weaver
Jan 1, 1997·Calcified Tissue International·J D Ringe
Nov 19, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Husheem MichaelTeuvo A Hentunen
Sep 16, 2000·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·M Adelina Costa, M Helena Fernandes
May 27, 2004·Technology in Cancer Research & Treatment·Seema GuptaViney Jain
Mar 3, 2004·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Y YamanakaT Maruo
Jan 12, 2001·Physiological Reviews·J E Compston

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