17 Beta-hydroxysteroid UDP-glucuronosyl transferase is expressed in bile ductular epithelial cells under physiological conditions

Biochemical and Biophysical Research Communications
K L FransonG A Mathis

Abstract

Using an improved procedure to isolate pure bile ductular epithelial cells from rat liver, we were able to define UDP-glucuronosyl transferase (UDPGT) isozymes expressed in these cells under physiological conditions. The cells contained mRNA for a 17 beta-hydroxysteroid, a 3 alpha-hydroxysteroid and a phenol UDPGT. Concomitantly, a distinct pattern of UDPGT-immunoreactive proteins was expressed, including a putative 17 beta-hydroxysteroid UDPGT. The presence of this UDPGT isozyme was confirmed by a high level of testosterone glucuronidation activity. This is the first demonstration of a metabolic pathway in bile ductular epithelial cells that is primarily dedicated to the processing of endogenous compounds.

References

Jul 1, 1976·Analytical Biochemistry·G S RaoH Breuer
Jul 1, 1990·Trends in Pharmacological Sciences·T R Tephly, B Burchell
Jan 1, 1990·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·A E SiricaL W Elmore
Feb 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·G A Mathis, A E Sirica
Jan 1, 1989·Pharmacology & Therapeutics·B Burchell, M W Coughtrie
Mar 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G A MathisA E Sirica
Jan 15, 1987·Biochemical Pharmacology·M W CoughtrieJ R Bend
Mar 1, 1987·The Biochemical Journal·M R JacksonB Burchell
Nov 1, 1988·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·T TephlyY Irshaid
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D A Goldberg
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L M Hjelmeland

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Citations

Jan 1, 1991·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·R LenziN Tavoloni

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