17-DMAG-loaded nanofibrous scaffold for effective growth inhibition of lung cancer cells through targeting HSP90 gene expression

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Hassan MellatyarNosratollah Zarghami

Abstract

Up-regulation of heat shock protein 90 (HSP90) gene takes place in lung cancer cells. Therefore, targeting HSP90 in lung cancer may be promising step in lung cancer therapy. The present study aimed to evaluate the efficiency of implantable 17-dimethylaminoethylamino-17-demethoxy geldanamycin (17-DMAG)-loaded Poly(caprolactone)-poly(ethylene glycol) (PCL/PEG) nanofibers to increase the anti-cancer effects via inhibition of HSP90 expression and telomerase activity. For this purpose, 17-DMAG-loaded PCL/PEG nanofibers were successfully fabricated via electrospinning and characterized using FE-SEM and FTIR. Colorimetric MTT assay was used to determine the drug cytotoxicity. Also, the expression levels of HSP90 mRNA in the A549 cells treated with the nanofibers were assessed using Quantitative Real-Time PCR. The effect of free 17-DMAG and 17-DMAG-loaded PCL/PEG nanofiber treatment on telomerase activity was monitored by TRAP assay. MTT assay confirmed that loading of 17-DMAG into PCL/PEG nanofiber enhanced dramatically cytotoxicity in the lung cancer cells. This finding was associated with reduction of HSP90 mRNA expression and telomerase activity in the cells seeded on 17-DMAG-loaded PCL/PEG nanofibers in relative to free 17-DMAG. I...Continue Reading

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Mar 31, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hassan MellatyarNosratollah Zarghami

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Citations

Jan 31, 2019·Chemical Biology & Drug Design·Sona TalaeiNosratollah Zarghami
Jan 25, 2020·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Archana A GuptaPeter A Brennan
Apr 5, 2020·Journal of Biomedical Materials Research. Part a·Meysam KhodadadiYounes Pilehvar-Soltanahmadi
Apr 17, 2019·Nanomaterials·S M Shatil ShahriarYong-Kyu Lee
May 14, 2020·Mini Reviews in Medicinal Chemistry·Mohsen DoostmohammadiSeeram Ramakrishna
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Shadi SamadzadehHanieh Mousazadeh

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