17β-estradiol ameliorates oxidative stress and blue light-emitting diode-induced retinal degeneration by decreasing apoptosis and enhancing autophagy

Drug Design, Development and Therapy
Qingquan WeiJing Yu

Abstract

This study aimed to assess the effects of 17β-estradiol (βE2) on blue light-emitting diode (LED)-induced retinal degeneration (RD) in rats and hydrogen peroxide (H2O2)-induced retinal pigment epithelium cell injury in humans and elucidate the protective mechanism of βE2 underlying these processes. Female ovariectomized (OVX) rats were intravitreally injected with βE2 before blue LED exposure (3,000 lux, 2 hours). Retinal function and morphology were assayed via electroretinogram (ERG) and H&E, respectively. Cell viability was assayed using the Cell Counting Kit-8. Cell ROS were measured using dichlorofluorescein fluorescence. Apoptosis was evaluated by TUNEL and Annexin V/propidium iodide staining. Gene expression and protein expression were quantified using quantitative real-time RT-PCR, Western blotting, and immunohistochemistry. Autophagosomes were examined by electron microscopy. Female OVX rats were exposed to blue LED, inducing RD. βE2 significantly prevented the reduction in the a- and b-wave ERG amplitudes and the disruption of retinal structure, the loss of photoreceptor cells, and the decrease in the thickness of the outer nuclear layer caused by blue LED exposure. βE2 also decreased cell apoptosis in the retina in bl...Continue Reading

Citations

Aug 25, 2020·Oxidative Medicine and Cellular Longevity·Zi-Yuan ZhangGuang-Yu Li
Feb 26, 2021·Oxidative Medicine and Cellular Longevity·Cemal OrhanKazim Sahin
Mar 7, 2021·Life·José A Fernández-AlbarralAna I Ramírez
Aug 9, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xinli OuyangGuohui Wang

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Methods Mentioned

BETA
protein assay
PCR
flow cytometry

Software Mentioned

SPSS
CalcuSyn
Quantity One
- Pro Analyzer
Gel

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