17β-Estradiol modulates the prolactin secretion induced by TRH through membrane estrogen receptors via PI3K/Akt in female rat anterior pituitary cell culture

American Journal of Physiology. Endocrinology and Metabolism
Liliana d V SosaAlicia I Torres

Abstract

Considering that estradiol is a major modulator of prolactin (PRL) secretion, the aim of the present study was to analyze the role of membrane estradiol receptor-α (mERα) in the regulatory effect of this hormone on the PRL secretion induced by thyrotropin-releasing hormone (TRH) by focusing on the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) pathway activation. Anterior pituitary cell cultures from female rats were treated with 17β-estradiol (E(2), 10 nM) and its membrane-impermeable conjugated estradiol (E(2)-BSA, 10 nM) alone or coincubated with TRH (10 nM) for 30 min, with PRL levels being determined by RIA. Although E(2), E(2)-BSA, TRH, and E(2)/TRH differentially increased the PRL secretion, the highest levels were achieved with E(2)-BSA/TRH. ICI-182,780 did not modify the TRH-induced PRL release but significantly inhibited the PRL secretion promoted by E(2) or E(2)-BSA alone or in coincubation with TRH. The PI3K inhibitors LY-294002 and wortmannin partially inhibited the PRL release induced by E(2)-BSA, TRH, and E(2)/TRH and totally inhibited the PRL levels stimulated by E(2)-BSA/TRH, suggesting that the mER mediated the cooperative effect of E(2) on TRH-induced PRL release through the PI3K pathway. Also, t...Continue Reading

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Citations

May 26, 2017·Journal of Cellular Physiology·Pablo A PerezSilvina Gutierrez
Feb 23, 2020·Journal of Toxicology and Environmental Health. Part a·Katriane Neto da SilvaViviane Glaser
Feb 23, 2020·Reproduction in Domestic Animals = Zuchthygiene·Wioleta CzelejewskaStanislaw Okrasa
May 9, 2013·American Journal of Physiology. Endocrinology and Metabolism·Liliana del V SosaAlicia I Torres
Mar 27, 2020·OncoTargets and Therapy·Yanfang LiuJing Yao

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