17β-Estradiol regulates scavenger receptor class BI gene expression via protein kinase C in vascular endothelial cells

Endocrine
Youko FukataKoji Murao

Abstract

High-density lipoprotein (HDL) mediates reverse cholesterol transport. In this process, the human homolog of the B class, type I scavenger receptor (SR-BI), CD36, and LIMPII analogous-1 (hSR-BI/CLA-1) facilitates the cellular uptake of cholesterol from HDL. In endothelial cells, HDL activates endothelial nitric oxide synthase (eNOS) via hSR-BI/CLA-1, and 17β-estradiol (E2) modulates nitric oxide (NO) synthesis. In this study, we elucidated the effect of E2 on hSR-BI/CLA-1 expression in human umbilical vein endothelial cells (HUVECs). HSR-BI/CLA-1 expression was examined by real-time PCR, western blot analysis and reporter gene assay in HUVECs incubated with E2. eNOS activity was assessed by detection of phosphorylation (Ser 1179) of eNOS. We investigated the effect of the constitutively active form or dominant negative form of protein kinase C on hSR-BI/CLA-1 promoter activity. Our results showed that E2 increased the endogenous expression of hSR-BI/CLA-1. E2 also enhanced the activity of the hSR-BI/CLA-1 promoter and the expression of its mRNA. However, bisindolylmaleimide I, an inhibitor of protein kinase C, blocked the stimulatory effect of E2 on hSR-BI/CLA-1 promoter activity. Moreover, constitutively active PKC increased t...Continue Reading

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Citations

Oct 25, 2016·Frontiers in Pharmacology·Jorge L Gutierrez-PajaresPhilippe G Frank
Jan 5, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Xiao YuJing-Wei Chen
Mar 17, 2017·Current Opinion in Lipidology·Menno Hoekstra, Mary Sorci-Thomas
Sep 28, 2018·Frontiers in Pharmacology·Waranya ChatuphonprasertIsabella Ellinger

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