17beta-Estradiol enhances neuronal differentiation of mouse embryonic stem cells

FEBS Letters
Alexander K MurashovLisa M Tatko

Abstract

Existing protocols show a variety in the percentage of neurons that can be generated from mouse embryonic stem (ES) cells. In the current study, we compared effects of various differentiating conditions, including gelatin and poly-l-ornithine/fibronectin coatings, and NGF and 17beta-estradiol treatments on the total yield of neurons, as well as, neurite growth and branching. Here, we show that combination of fibronectin coating with 17beta-estradiol increased number of generated neurons over 50%. Poly-l-ornithine/fibronectin increased the percent of neurons in all cultures, suggesting its direct influence on neurogenesis. Addition of 17beta-estradiol reduced mean neurite length in culture, but significantly increased branching. Our results indicate a substrate-dependent regulation of estrogen-induced ES cells differentiation into neuronal cells.

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Citations

May 17, 2005·Biochemical and Biophysical Research Communications·Alexander K MurashovLaxmansa C Katwa
May 14, 2008·Molecular Medicine·Rinki RayDaniel R Meldrum
Jan 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Cassandra D KinchDeborah M Kurrasch
Oct 29, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Helmut Segner
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Sep 22, 2016·Neural Regeneration Research·Ricardo Ramírez-BarrantesPablo Olivero
Oct 11, 2016·Molecular and Cellular Endocrinology·Dinushan Nesan, Deborah M Kurrasch
Dec 13, 2005·Biochemical and Biophysical Research Communications·Mi Hee ParkJin Tae Hong
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Jul 8, 2014·Cell Medicine·Mibel PabonCesar V Borlongan
Oct 6, 2007·Endocrinology·Denise R Goldman-JohnsonJohn R Morrison
Nov 19, 2019·Translational Neuroscience·Pelin Kelicen-UgurHande Karahan
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Feb 2, 2010·Frontiers in Neuroendocrinology·Nicolas DiotelOlivier Kah

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