17beta-estradiol enhances the production of granulocyte-macrophage colony-stimulating factor in human keratinocytes

The Journal of Investigative Dermatology
Naoko Kanda, Shinichi Watanabe

Abstract

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is effective for impaired wound repair. Estrogen is known to enhance wound repair. We examined if 17beta-estradiol (E2) may in vitro enhance GM-CSF production in human keratinocytes. E2 and membrane-impermeable bovine serum albumin-conjugated E2 increased GM-CSF secretion, mRNA stability, and promoter activity. The element homologous to activator protein-1 (AP-1) on the promoter was responsible for the activation. E2 enhanced transcriptional activity and DNA binding of AP-1. E2 transiently generated c-Fos protein, and shifted AP-1 composition from c-Jun homodimers to c-Fos/c-Jun heterodimers in keratinocytes. E2-induced enhancement of GM-CSF secretion, mRNA stability, and promoter activity were not suppressed by estrogen receptor antagonist ICI 182,780, however, suppressed by conventional protein kinase C inhibitor Gö6976 and PD98059, an inhibitor of mitogen-activated protein kinase kinase (MEK). Gö6976 and PD98059 suppressed E2-induced c-Fos expression and enhancement of DNA-binding and transcriptional activity at AP-1. E2 induced membrane translocation of protein kinase Calpha, which was suppressed by phosphatidylinositol (PI)-specific phospholipase C (PLC) inhibitor U...Continue Reading

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Citations

Dec 20, 2012·Physiological Genomics·Michael C Velarde
Jun 10, 2010·Zoological Science·Tomohisa HirobeShosuke Ito
Mar 30, 2005·Journal of Dermatological Science·Naoko Kanda, Shinichi Watanabe
Jun 14, 2005·Expert Opinion on Therapeutic Targets·M J Thornton
Feb 26, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Andrzej SlominskiRobert C Tuckey
Oct 21, 2016·Reviews in Endocrine & Metabolic Disorders·Georgios NikolakisChristos C Zouboulis
Mar 12, 2005·American Journal of Physiology. Cell Physiology·Naoko Kanda, Shinichi Watanabe
Aug 1, 2006·Endocrine Reviews·Ulrich OhnemusRalf Paus
Jan 24, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gertrude-E Costin, Vincent J Hearing
Oct 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Allen MaoSusan Kovats
Jul 31, 2019·International Journal of Molecular Sciences·Andrea Olmos-OrtizVerónica Zaga-Clavellina
Sep 28, 2021·Immunological Investigations·Katia Jarquín-YáñezAndrés Eliú Castell-Rodríguez

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