17beta-estradiol induced vitellogenesis is inhibited by cortisol at the post-transcriptional level in Arctic char (Salvelinus alpinus)

Reproductive Biology and Endocrinology : RB&E
Hakan BergPer-Erik Olsson

Abstract

This study was performed to investigate stress effects on the synthesis of egg yolk precursor, vitellogenin (Vtg) in Arctic char (Salvelinus alpinus). In particular the effect of cortisol (F) was determined since this stress hormone has been suggested to interfere with vitellogenesis and is upregulated during sexual maturation in teleosts. Arctic char Vtg was purified and polyclonal antibodies were produced in order to develop tools to study regulation of vitellogenesis. The Vtg antibodies were used to develop an enzyme-linked immunosorbent assay. The corresponding Vtg cDNA was cloned from a hepatic cDNA library in order to obtain DNA probes to measure Vtg mRNA expression. Analysis of plasma from juvenile Arctic char, of both sexes, exposed to different steroids showed that production of Vtg was induced in a dose dependent fashion by 17beta-estradiol (E2), estrone and estriol. Apart from estrogens a high dose of F also upregulated Vtg. In addition, F, progesterone (P) and tamoxifen were tested to determine these compounds ability to modulate E2 induced Vtg synthesis at both the mRNA and protein level. Tamoxifen was found to inhibit E2 induced Vtg mRNA and protein upregulation. P did not alter the Vtg induction while F reduced t...Continue Reading

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Citations

May 20, 2015·Integrative and Comparative Biology·Melissa S LammJohn R Godwin
Apr 4, 2009·General and Comparative Endocrinology·Neelakanteswar Aluru, Mathilakath M Vijayan
Aug 27, 2010·Journal of Fish Biology·J F LeatherlandS Barkataki
Mar 19, 2014·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Ambarisha Chabbi, C B Ganesh
Jul 29, 2019·Bulletin of Environmental Contamination and Toxicology·Clau John A MabansagMa Suzanneth Epifania G Lola
Jan 31, 2013·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Ambarisha Chabbi, C B Ganesh
Oct 13, 2020·General and Comparative Endocrinology·Karine RousseauSylvie Dufour
Dec 6, 2005·Environmental Science & Technology·Zhiqiang ZengZhiyuan Gong

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Methods Mentioned

BETA
electrophoresis
enzyme-linked immunosorbent assay
ELISA
dot blot
PCR

Software Mentioned

Quantity One
GraphPad Prism
GraphPad

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