17beta-Estradiol promotes striatal medium size spiny neuronal maturation in vitro

Neuroendocrinology
Antonella StroppoloMichelle E Ehrlich

Abstract

Gender differences exist in the development of the nigrostriatal dopamine system, and in the incidence and course of pediatric and adult neuropsychiatric diseases in which this system is implicated. The medium size spiny neuron (MSN) is the major output neuron of the caudate nucleus. It receives a large dopaminergic input from the substantia nigra, and 96% of the MSNs express DARPP-32, a dopamine and cyclic AMP-regulated phosphoprotein and key mediator of dopamine function. There are few examples, however, of direct effects of sex hormones, including 17beta-estradiol (E(2)), on the MSN. We report that in vitro, E(2) (10-50 nM) promotes MSN phenotypic maturation, as determined by increased soma size, neurite length, and DARPP-32 protein levels. Treatment with the 'anti-estrogen' ICI 182,780 or the partial-agonist tamoxifen also increases DARPP-32 levels, but when added to E(2), ICI 182,780 only prevents the increase in DARPP-32 levels and increase in soma size and neurite length. Surprisingly, maturation effects are more robust in cells derived exclusively from female embryos. Western blot analysis of protein lysates and immunocytochemistry of cultured MSNs reveals the presence of the estrogen receptor beta (ERbeta). These data ...Continue Reading

Citations

May 2, 2012·International Journal of Alzheimer's Disease·Richelin V DyeAndrew J Levine
Apr 15, 2005·Genesis : the Journal of Genetics and Development·Alexey I BogushMichelle E Ehrlich
Nov 28, 2007·Journal of Neuroscience Research·Junghee LeeHoon Ryu
Dec 2, 2011·Journal of Neuroendocrinology·C E Roselli, F Stormshak
Nov 4, 2005·Current Opinion in Neurobiology·Constance Scharff, Sebastian Haesler
Oct 6, 2005·Neuroscience Letters·Christopher SaenzSonsoles de Lacalle
Jun 7, 2011·Neurotoxicology·Jennifer L MadisonAaron B Bowman
Jan 9, 2007·The Journal of Biological Chemistry·Alexey BogushMichelle E Ehrlich

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