17beta-estradiol rapidly mobilizes intracellular calcium from ryanodine-receptor-gated stores via a PKC-PKA-Erk-dependent pathway in the human eccrine sweat gland cell line NCL-SG3

Cell Calcium
Ruth W Muchekehu, Brian J Harvey

Abstract

We describe a novel rapid non-genomic effect of 17beta-estradiol (E2) on intracellular Ca2+ ([Ca2+]i) signalling in the eccrine sweat gland epithelial cell line NCL-SG3. E2 had no observable effect on basal [Ca2+]i, however exposure of cells to E2 in the presence of the microsomal Ca2+ ATPase pump inhibitor, thapsigargin, produced a secondary, sustained increase in [Ca2+]i compared to thapsigargin treatment alone, where cells responded with a transient single spike-like increase in [Ca2+]i. The E2-induced increase in [Ca2+]i was not dependent on the presence of extracellular calcium and was completely abolished by ryanodine (100 microM). The estrogen receptor antagonist ICI 182,780 (1 microM) prevented the E2-induced effects suggesting a role for the estrogen receptor in the release of [Ca2+]i from ryanodine-receptor-gated stores. The E2-induced effect on [Ca2+]i could also be prevented by the protein kinase C delta (PKCdelta)-specific inhibitor rottlerin (10 microM), the protein kinase A (PKA) inhibitor Rp-adenosine 3',5'-cyclic monophosphorothioate (200 microM) and the MEK inhibitor PD98059 (10 microM). We established E2 rapidly activates the novel PKC isoform PKCepsilon, PKA and Erk 1/2 MAPK in a PKCdelta and estrogen-recept...Continue Reading

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Citations

Jan 4, 2012·Taiwanese Journal of Obstetrics & Gynecology·Kuan-Hao TsuiHsin-Yang Li
Nov 26, 2009·Pharmacology & Therapeutics·Isaac N PessahPamela J Lein
Jun 16, 2015·Environment International·Ryoiti Kiyama, Yuko Wada-Kiyama
Jun 16, 2009·Molecular and Cellular Endocrinology·A M HoganD C Winter
Feb 20, 2009·Cell Biochemistry and Function·Ludmila ZylinskaBozena Ferenc
Nov 11, 2009·Journal of Receptor and Signal Transduction Research·Volodymyr RybalchenkoPeter Koulen
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Oct 18, 2011·Trends in Cell Biology·Michael MaceykaSarah Spiegel
Aug 8, 2018·Journal of Molecular Recognition : JMR·Guido CaluoriVladimir Rotrekl
Jul 25, 2017·Environmental Science and Pollution Research International·Bingli LeiWei Peng
Apr 20, 2019·Frontiers in Cellular Neuroscience·Lucas E Cabrera ZapataMaría Julia Cambiasso

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