18 F]BODIPY-triglyceride-containing chylomicron-like particles as an imaging agent for brown adipose tissue in vivo

Scientific Reports
Andreas PaulusMatthias Bauwens

Abstract

Brown adipose tissue (BAT) is present in human adults and the current gold standard to visualize and quantify BAT is [18F]FDG PET-CT. However, this method fails to detect BAT under insulin-resistant conditions associated with ageing and weight gain, such as type 2 diabetes. The aim of this study was to develop a novel triglyceride-based tracer for BAT. For this purpose we designed a dual-modal fluorescent/PET fatty acid tracer based on commercially available BODIPY-FL-C16, which can be esterified to its correspondent triglyceride, radiolabeled and incorporated into pre-synthesized chylomicron-like particles. BODIPY-FL-C16 was coupled to 1,2-diolein with a subsequent radiolabeling step resulting in [18F]BODIPY-C16-triglyceride that was incorporated into chylomicron-like particles. Various quality control steps using fluorescent and radioactive methods were conducted before BAT visualization was tested in mice. Triglyceride synthesis, radiolabeling and subsequent incorporation into chylomicron-like particles was carried out in decent yields. This radiotracer appeared able to visualize BAT in vivo, and the uptake of the radiotracer was stimulated by cold exposure. The here reported method can be used to incorporate radiolabeled tr...Continue Reading

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Citations

Apr 10, 2020·The Biochemical Journal·Marie Anne RichardDenis P Blondin
Dec 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maxime MunchGilles Ulrich
Jun 21, 2020·Chemistry and Physics of Lipids·Liliana Aranda-LaraKeila Isaac-Olivé
Apr 30, 2021·Molecular Pharmaceutics·Mohammad Sharifian Gh
Apr 24, 2021·Molecular Metabolism·Joerg Heeren, Ludger Scheja

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Methods Mentioned

BETA
NMR
transmission electron microscopy
electrophoresis
Fluorescence

Software Mentioned

GraphPad Prism
CONTIN

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