1alpha,25-Dihydroxyvitamin D3 modulates human adipocyte metabolism via nongenomic action

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
H ShiM B Zemel

Abstract

We reported recently that suppression of the renal 1alpha,25-dihyroxyvitamin D3 (1lpha,25-(OH)2-D3) production in aP2-agouti transgenic mice by increasing dietary calcium decreases adipocyte intracellular Ca2+ ([Ca2+]i), stimulates lipolysis, inhibits lipogenesis, and reduces adiposity. However, it was not clear whether this modulation of adipocyte metabolism by dietary calcium is a direct effect of inhibition of 1alpha,25-(OH)2-D3-induced [Ca2+]i. Accordingly, we have now evaluated the direct role of 1alpha,25-(OH)2-D3. Human adipocytes exhibited a 1alpha,25-(OH)2-D3 dose-responsive (1-50 nM) increase in [Ca2+]i (P<0.01). This action was mimicked by 1alpha,25-dihyroxylumisterol3 (1alpha,25-(OH)2-lumisterol3) (P<0.001), a specific agonist for a putative membrane vitamin D receptor (mVDR), and completely prevented by 1b,25-dihydroxyvitamin D3 (1beta,25-(OH)2-D3), a specific antagonist for the mVDR. Similarly, 1alpha,25-(OH)2-D3 (5 nM) caused 50%-100% increases in adipocyte fatty acid synthase (FAS) expression and activity (P<0.02), a 61% increase in glycerol-3-phosphate dehydrogenase (GPDH) activity (P<0.01), and an 80% inhibition of isoproterenol-stimulated lipolysis (P<0.001), whereas 1beta,25-(OH)2-D3 completely blocked all t...Continue Reading

Associated Clinical Trials

References

Jul 1, 1992·American Journal of Hypertension·R L ByynyB Draznin
Dec 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D T BaranJ Lian
Apr 1, 1991·Endocrinology·A S DussoA J Brown
Oct 1, 1988·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N H BellS Shaw
Jul 1, 1985·The Journal of Clinical Investigation·N H BellS Shaw
May 1, 1988·Metabolism: Clinical and Experimental·T AndersenI Transbøl
Apr 1, 1986·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S EpsteinM J Oexmann
Aug 15, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·L H Boobis, R J Maughan
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·M B ZemelW O Wilkison
Apr 1, 1995·Endocrine Reviews·R BouillonA W Norman
Mar 1, 1997·The American Journal of Physiology·J H KimM B Zemel
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y C LiM B Demay
May 2, 1998·Nutrition Reviews·H F DeLuca, C Zierold
Sep 17, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·I NemereB D Boyan
Sep 17, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A W Norman
Mar 18, 1999·Nutrition Reviews·J C Fleet
Oct 3, 1999·Progress in Cardiovascular Diseases·L Resnick
Oct 3, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H ShiM B Zemel
Oct 4, 2000·The Journal of Nutrition·B Xue, M B Zemel
Oct 4, 2000·Physiological Genomics·H ShiM B Zemel
Oct 20, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B XueM B Zemel

❮ Previous
Next ❯

Citations

Mar 18, 2010·Archives of Osteoporosis·G Amarendra ReddyK Usha Rani
Dec 6, 2011·Journal of Physiology and Biochemistry·Natalia El-MerhieMahmoud Balbaa
Jun 27, 2012·Journal of Women's Health·Erin S LeBlancUNKNOWN Study Of Osteoporotic Fractures
Jul 25, 2012·Human Reproduction·Janelle LukLubna Pal
Jan 1, 2008·Journal of Nutrigenetics and Nutrigenomics·Taru K PilviEero M Mervaala
May 19, 2012·Endocrine Reviews·Clifford J RosenChristopher S Kovacs
Oct 3, 2013·Nutrients·Meis Moukayed, William B Grant
Oct 9, 2013·Calcified Tissue International·Mark E NuttallJeffrey M Gimble
Mar 25, 2014·International Journal of Molecular Sciences·Mario J SoaresEmily K Calton
Dec 5, 2008·Nutrition Reviews·Michael B Zemel, Xiaocun Sun
Feb 7, 2004·The Journal of Clinical Endocrinology and Metabolism·Sue A ShapsesSteven B Heymsfield
Mar 6, 2008·European Journal of Nutrition·Steinar KonradsenRolf Jorde
Jun 28, 2011·European Journal of Nutrition·Silvia Lorente-CebriánMikael Rydén
Apr 28, 2012·European Journal of Nutrition·Tuija JääskeläinenRitva Järvinen
Sep 20, 2012·Journal of Physiology and Biochemistry·Mona A Ahmed
May 27, 2014·Frontiers in Physiology·Wei-Lin W Wang, Martin Tenniswood
Jun 22, 2016·The Journal of Endocrinology·E P S ConceiçãoP C Lisboa
Sep 18, 2007·The Journal of Nutritional Biochemistry·Xiaocun Sun, Michael B Zemel
Oct 9, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Jia LiDorothy Teegarden
Nov 23, 2006·Arquivos brasileiros de endocrinologia e metabologia·Robert P Heaney
Feb 2, 2017·Nutrients·Victoria G RontoyanniSreenivas P Veeranki
Jan 4, 2018·International Journal of Environmental Research and Public Health·Joanna KocotIrena Musik
Apr 7, 2007·The Journal of Craniofacial Surgery·Sanna-Mari NiemeläTimo Ylikomi
Feb 12, 2005·The British Journal of Nutrition·Gianvincenzo BarbaAlfonso Siani
Aug 19, 2007·The British Journal of Nutrition·Taru K PilviEero M Mervaala
Feb 20, 2008·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·G C MajorA Tremblay
Apr 23, 2008·International Journal of Obesity : Journal of the International Association for the Study of Obesity·X Sun, M B Zemel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Related Papers

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Hang ShiM B Zemel
FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
B XueM B Zemel
Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme
Oliver C WitardKevin D Tipton
Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Nasser M Al-DaghriShaun Sabico
Nature Reviews. Endocrinology
R BouillonA Verstuyf
© 2021 Meta ULC. All rights reserved