1alpha,25(OH)2D3 and parathyroid hormone (PTH) signaling in rat intestinal cells: activation of cytosolic PLA2

The Journal of Steroid Biochemistry and Molecular Biology
Claudia GentiliA R de Boland

Abstract

In the current study, we have probed the role of cytosolic phospholipase A2 (cPLA2) activity in the cellular response to the calciotropic hormones, 1alpha,25,dihydroxy-vitamin D(3) [1alpha,25(OH)(2)D(3)] and PTH. Stimulation of rat enterocytes with either hormone, increased release of arachidonic acid (AA) 3H-AA] one-two fold in a concentration and time-dependent manner. The effect of either hormone on enterocytes was totally reduced by preincubation with the intracellular Ca(2+) chelator BAPTA-AM (5 microM), suggesting that the release of AA following cell exposure to the calciotropic hormones occurs mainly through a Ca(2+)-dependent mechanism involving activation of Ca(2+)-dependent cPLA2. Calciotropic homone stimulation of rat intestinal cells increases cPLA2 phosphorylation (three to four fold). This effect was decreased by PD 98059 (20 microM), a MAP kinase inhibitor, indicating that this action is, in part, mediated through activation of the MAP kinases ERK 1 and ERK2. Enterocytes exposure to 1alpha,25(OH)(2)D(3) (1nM) or PTH (10 nM) also resulted in P-cPLA2 translocation from cytosol to nuclei and membrane fractions, where phospholipase subtrates reside. Collectively, these data suggest that PTH and 1alpha,25(OH)(2)D(3) ...Continue Reading

References

Mar 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·A W NormanM C Farach-Carson
May 1, 1992·Journal of Cellular Biochemistry·A R de Boland, I Nemere
Jan 29, 1993·Cell·L L LinR J Davis
Jul 1, 1997·Kidney International·J N SheuR Quigley
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Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Ichiro Kudo, Makoto Murakami
Feb 13, 2003·Biochimica Et Biophysica Acta·Claudia GentiliAna Russo de Boland

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Citations

Apr 17, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Natalia CalvoClaudia Gentili
Nov 27, 2004·Mechanisms of Ageing and Development·Ana Russo de Boland

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